BMRB Entry 52141

Name: Backbone 1H, 13C,and 15N chemical shift assignments for human SERF2
Published: 2024-03-11

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52141

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Backbone 1H, 13C,and 15N chemical shift assignments for human SERF2 PubMed: 38466543

Deposition date: 2023-09-19 Original release date: 2024-03-11

Authors: Sahoo, Bikash; Subramanian, Vivekanandan; Bardwell, James

Citation: Sahoo, Bikash; Subramanian, Vivekanandan; Bardwell, James. "Backbone 1H, 13C, and 15N chemical shift assignments for human SERF2" Biomol. NMR Assign. ., .-. (2024).

Assembly members:
entity_1, polymer, 59 residues, Formula weight is not available

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28

Entity Sequences (FASTA):
entity_1: MTRGNQRELARQKNMKKQSD SVKGKRRDDGLSAAARKQRD SEIMQQKQKKANEKKEEPK

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	150
15N chemical shifts	50
1H chemical shifts	278

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	human SERF2	1

Entities:

Entity 1, human SERF2 59 residues - Formula weight is not available

1

MET

THR

ARG

GLY

ASN

GLN

ARG

GLU

LEU

ALA

2

ARG

GLN

LYS

ASN

MET

LYS

GLN

SER

ASP

3

SER

VAL

LYS

GLY

LYS

ARG

ASP

GLY

4

LEU

SER

ALA

ARG

LYS

GLN

ARG

ASP

5

SER

GLU

ILE

MET

GLN

LYS

GLN

LYS

6

ALA

ASN

GLU

LYS

GLU

PRO

LYS

Samples:

sample_1: Human SERF2, [U-100% 13C; U-100% 15N], 1.2 mM; d3-Sodium acetate 20 mM; KCl 20 mM; EDTA 2 mM

sample_conditions_1: ionic strength: 0.1 M; pH: 5.5; pressure: 1 atm; temperature: 277.1 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCANH	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1

Software:

NMRFAM-SPARKY v1.470 - chemical shift assignment

NMR spectrometers:

Bruker AVANCE NEO 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts