BMRB Entry 52142

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52142

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
1H, 15N and 13C chemical shift assignments for the D2 domain of the human DEAD-box RNA helicase DDX3X
Deposition date:
2023-09-19
Original release date:
2024-03-18
Authors:
Toyama, Yuki; Shimada, Ichio
Citation:

Citation: Toyama, Yuki; Shimada, Ichio. "NMR characterization of RNA binding property of the DEAD-box RNA helicase DDX3X and its implications for helicase activity"  Nat. Commun. 15, 3303-3303 (2024).
PubMed: 38664397

Assembly members:

Assembly members:
entity_1, polymer, 201 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-SUMO

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GGGSTSENITQKVVWVEESD KRSFLLDLLNATGKDSLTLV FVETKKGADSLEDFLYHEGY ACTSIHGDRSQRDREEALHQ FRSGKSPILVATAVAARGLD ISNVKHVINFDLPSDIEEYV HRIGRTGRVGNLGLATSFFN ERNINITKDLLDLLVEAKQE VPSWLENMAYEHHYKGSSRG RSKSSRFSGGFGARDYRQSS G

Data sets:
Data typeCount
13C chemical shifts565
15N chemical shifts189
1H chemical shifts219

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1D2 domain1

Entities:

Entity 1, D2 domain 201 residues - Formula weight is not available

The first two Gly residues were introduced to facilitate the cleavage of N-terminal His-SUMO tag.

1   GLYGLYGLYSERTHRSERGLUASNILETHR
2   GLNLYSVALVALTRPVALGLUGLUSERASP
3   LYSARGSERPHELEULEUASPLEULEUASN
4   ALATHRGLYLYSASPSERLEUTHRLEUVAL
5   PHEVALGLUTHRLYSLYSGLYALAASPSER
6   LEUGLUASPPHELEUTYRHISGLUGLYTYR
7   ALACYSTHRSERILEHISGLYASPARGSER
8   GLNARGASPARGGLUGLUALALEUHISGLN
9   PHEARGSERGLYLYSSERPROILELEUVAL
10   ALATHRALAVALALAALAARGGLYLEUASP
11   ILESERASNVALLYSHISVALILEASNPHE
12   ASPLEUPROSERASPILEGLUGLUTYRVAL
13   HISARGILEGLYARGTHRGLYARGVALGLY
14   ASNLEUGLYLEUALATHRSERPHEPHEASN
15   GLUARGASNILEASNILETHRLYSASPLEU
16   LEUASPLEULEUVALGLUALALYSGLNGLU
17   VALPROSERTRPLEUGLUASNMETALATYR
18   GLUHISHISTYRLYSGLYSERSERARGGLY
19   ARGSERLYSSERSERARGPHESERGLYGLY
20   PHEGLYALAARGASPTYRARGGLNSERSER
21   GLY

Related Database Links:

UNP O00571

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks