BMRB Entry 52153

Title:
Endo-b-1,4-xylanase (Xylanase A) D11F/R122D mutant from Bacillus subtilis
Deposition date:
2023-09-28
Original release date:
2023-10-18
Authors:
MacDonald, Meagan
Citation:

Citation: MacDonald, Meagan; Wells, Nicholas; Hassan, Bakar; Dudley, Joshua; Walters, Kylie; Korzhnev, Dmitry; Aramini, James; Smith, Colin. "Effects of Xylanase A double mutation on substrate specificity and structural dynamics"  J. Struct. Biol. 216, 108082-108082 (2024).
PubMed: 38438058

Assembly members:

Assembly members:
entity_1, polymer, 189 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Bacillus subtilis   Taxonomy ID: 1423   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Bacillus subtilis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET15b

Data sets:
Data typeCount
13C chemical shifts338
15N chemical shifts160
1H chemical shifts160

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Xylanase A, D11F/R122D1

Entities:

Entity 1, Xylanase A, D11F/R122D 189 residues - Formula weight is not available

His-tag scar GSHM included in the sequence

1   GLYSERHISMETALASERTHRASPTYRTRP
2   GLNASNTRPTHRPHEGLYGLYGLYILEVAL
3   ASNALAVALASNGLYSERGLYGLYASNTYR
4   SERVALASNTRPSERASNTHRGLYASNPHE
5   VALVALGLYLYSGLYTRPTHRTHRGLYSER
6   PROPHEARGTHRILEASNTYRASNALAGLY
7   VALTRPALAPROASNGLYASNGLYTYRLEU
8   THRLEUTYRGLYTRPTHRARGSERPROLEU
9   ILEGLUTYRTYRVALVALASPSERTRPGLY
10   THRTYRARGPROTHRGLYTHRTYRLYSGLY
11   THRVALLYSSERASPGLYGLYTHRTYRASP
12   ILETYRTHRTHRTHRARGTYRASNALAPRO
13   SERILEASPGLYASPASPTHRTHRPHETHR
14   GLNTYRTRPSERVALARGGLNSERLYSARG
15   PROTHRGLYSERASNALATHRILETHRPHE
16   SERASNHISVALASNALATRPLYSSERHIS
17   GLYMETASNLEUGLYSERASNTRPALATYR
18   GLNVALMETALATHRGLUGLYTYRGLNSER
19   SERGLYSERSERASNVALTHRVALTRP

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks