BMRB Entry 52153

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52153

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Title:
Endo-b-1,4-xylanase (Xylanase A) D11F/R122D mutant from Bacillus subtilis
Deposition date:
2023-09-28
Original release date:
2023-10-18
Authors:
MacDonald, Meagan
Citation:

Citation: MacDonald, Meagan; Wells, Nicholas; Hassan, Bakar; Dudley, Joshua; Walters, Kylie; Korzhnev, Dmitry; Aramini, James; Smith, Colin. "Effects of Xylanase A double mutation on substrate specificity and structural dynamics"  J. Struct. Biol. 216, 108082-108082 (2024).
PubMed: 38438058

Assembly members:

Assembly members:
entity_1, polymer, 189 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Bacillus subtilis   Taxonomy ID: 1423   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Bacillus subtilis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GSHMASTDYWQNWTFGGGIV NAVNGSGGNYSVNWSNTGNF VVGKGWTTGSPFRTINYNAG VWAPNGNGYLTLYGWTRSPL IEYYVVDSWGTYRPTGTYKG TVKSDGGTYDIYTTTRYNAP SIDGDDTTFTQYWSVRQSKR PTGSNATITFSNHVNAWKSH GMNLGSNWAYQVMATEGYQS SGSSNVTVW

Data sets:
Data typeCount
13C chemical shifts338
15N chemical shifts160
1H chemical shifts160

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1Xylanase A, D11F/R122D1

Entities:

Entity 1, Xylanase A, D11F/R122D 189 residues - Formula weight is not available

His-tag scar GSHM included in the sequence

1   GLYSERHISMETALASERTHRASPTYRTRP
2   GLNASNTRPTHRPHEGLYGLYGLYILEVAL
3   ASNALAVALASNGLYSERGLYGLYASNTYR
4   SERVALASNTRPSERASNTHRGLYASNPHE
5   VALVALGLYLYSGLYTRPTHRTHRGLYSER
6   PROPHEARGTHRILEASNTYRASNALAGLY
7   VALTRPALAPROASNGLYASNGLYTYRLEU
8   THRLEUTYRGLYTRPTHRARGSERPROLEU
9   ILEGLUTYRTYRVALVALASPSERTRPGLY
10   THRTYRARGPROTHRGLYTHRTYRLYSGLY
11   THRVALLYSSERASPGLYGLYTHRTYRASP
12   ILETYRTHRTHRTHRARGTYRASNALAPRO
13   SERILEASPGLYASPASPTHRTHRPHETHR
14   GLNTYRTRPSERVALARGGLNSERLYSARG
15   PROTHRGLYSERASNALATHRILETHRPHE
16   SERASNHISVALASNALATRPLYSSERHIS
17   GLYMETASNLEUGLYSERASNTRPALATYR
18   GLNVALMETALATHRGLUGLYTYRGLNSER
19   SERGLYSERSERASNVALTHRVALTRP

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