BMRB Entry 5218

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5218

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Title:
Backbone 1H, 13C, 15N resonance assignments of the N-terminal 24 kDa fragment of the gyrase B subunit from E. coli
Deposition date:
2001-11-23
Original release date:
2002-08-22
Authors:
Bellanda, Massimo; Peggion, Evaristo; Otting, Gottfried; Weigelt, Johan; Perdona, Elisabetta; Domenici, Enrico; Marchioro, Carla; Mammi, Stefano
Citation:

Citation: Bellanda, Massimo; Peggion, Evaristo; Otting, Gottfried; Weigelt, Johan; Perdona, Elisabetta; Domenici, Enrico; Marchioro, Carla; Mammi, Stefano. "Letter to the Editor: backbone 1H, 13C, 15N resonance assignments of the N-terminal 24 kDa fragment of the gyrase B subunit from E. coli "  J. Biomol. NMR 22, 369-370 (2002).
PubMed: 12018484

Assembly members:

Assembly members:
24 kDa N-terminal fragment of DNA Gyrase, polymer, 220 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pAM24

Entity Sequences (FASTA):

Entity Sequences (FASTA):
24 kDa N-terminal fragment of DNA Gyrase: MSNSYDSSSIKVLKGLDAVR KRPGMYIGDTDDGTGLHHMV FEVVDNAIDEALAGHCKEII VTIHADNSVSVQDDGRGIPT GIHPEEGVSAAEVIMTVLHA GGKFDDNSYKVSGGLHGVGV SVVNALSQKLELVIQREGKI HRQIYEHGVPQAPLAVTGET EKTGTMVRFWPSLETFTNVT EFEYEILAKRLRELSFLNSG VSIRLRDKRDGKEDHFHYEG

Data sets:
Data typeCount
1H chemical shifts403
13C chemical shifts551
15N chemical shifts196

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1GyraseB p241

Entities:

Entity 1, GyraseB p24 220 residues - Formula weight is not available

1   METSERASNSERTYRASPSERSERSERILE
2   LYSVALLEULYSGLYLEUASPALAVALARG
3   LYSARGPROGLYMETTYRILEGLYASPTHR
4   ASPASPGLYTHRGLYLEUHISHISMETVAL
5   PHEGLUVALVALASPASNALAILEASPGLU
6   ALALEUALAGLYHISCYSLYSGLUILEILE
7   VALTHRILEHISALAASPASNSERVALSER
8   VALGLNASPASPGLYARGGLYILEPROTHR
9   GLYILEHISPROGLUGLUGLYVALSERALA
10   ALAGLUVALILEMETTHRVALLEUHISALA
11   GLYGLYLYSPHEASPASPASNSERTYRLYS
12   VALSERGLYGLYLEUHISGLYVALGLYVAL
13   SERVALVALASNALALEUSERGLNLYSLEU
14   GLULEUVALILEGLNARGGLUGLYLYSILE
15   HISARGGLNILETYRGLUHISGLYVALPRO
16   GLNALAPROLEUALAVALTHRGLYGLUTHR
17   GLULYSTHRGLYTHRMETVALARGPHETRP
18   PROSERLEUGLUTHRPHETHRASNVALTHR
19   GLUPHEGLUTYRGLUILELEUALALYSARG
20   LEUARGGLULEUSERPHELEUASNSERGLY
21   VALSERILEARGLEUARGASPLYSARGASP
22   GLYLYSGLUASPHISPHEHISTYRGLUGLY

Samples:

sample_1: 24 kDa N-terminal fragment of DNA Gyrase, [U-13C; U-15N], 1.0 mM; sodium phosphate 20 mM; KCl 40 mM; NaCl 40 mM; EDTA 1 mM; DTT 2 mM; sodium azide 0.02 % w/v

sample_2: 24 kDa N-terminal fragment of DNA Gyrase, [U-75% 2H; U-13C; U-15N], 1.2 mM; sodium phosphate 10 mM; KCl 20 mM; EDTA 1 mM; DTT 2 mM; sodium azide 0.02 % w/v

Ex-cond_1: pH: 7.2; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions

Software:

No software information available

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Varian UnityPlus 800 MHz

Related Database Links:

PDB
DBJ BAA20341 BAB38057 BAC06612 BAC06613 BAC06614
EMBL CAA27871 CAP78158 CAQ34043 CAQ91428 CAR00672
GB AAA62050 AAG58896 AAN45208 AAN83054 AAP18989
PIR D86054
REF NP_290332 NP_312661 NP_709501 NP_756480 NP_839178
SP P0AES6 P0AES7 P0AES8
AlphaFold P0AES6 P0AES7 P0AES8

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks