BMRB Entry 52194
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52194
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Title: INCENP-complex PubMed: 38781028
Deposition date: 2023-10-26 Original release date: 2024-05-20
Authors: Sako, Kouske; Furukawa, Ayako; Nozawa, Ryu-Suke; Kurita, Jun-ichi; Nishimura, Yoshifumi; Hirota, Toru
Citation: Sako, Kosuke; Furukawa, Ayako; Nozawa, Ryu-Suke; Kurita, Jun-Ichi; Nishimura, Yoshifumi; Hirota, Toru. "Bipartite binding interface recruiting HP1 to chromosomal passenger complex at inner centromeres" J. Cell Biol. 223, e202312021-e202312021 (2024).
Assembly members:
entity_1, polymer, 33 residues, Formula weight is not available
entity_2, polymer, 81 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX6p-1
Entity Sequences (FASTA):
entity_1: HTQCQLVPVVEIGISERQNA
EQHVTQLMSTEPL
entity_2: NDIARGFERGLEPEKIIGAT
DSCGDLMFLMKWKDTDEADL
VLAKEANVKCPQIVIAFYEE
RLTWHAYPEDAENKEKETAK
S
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 24 |
| 1H chemical shifts | 24 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | INCENP | 1 |
| 2 | HP1_CSD | 2 |
Entities:
Entity 1, INCENP 33 residues - Formula weight is not available
| 1 | HIS | THR | GLN | CYS | GLN | LEU | VAL | PRO | VAL | VAL | ||||
| 2 | GLU | ILE | GLY | ILE | SER | GLU | ARG | GLN | ASN | ALA | ||||
| 3 | GLU | GLN | HIS | VAL | THR | GLN | LEU | MET | SER | THR | ||||
| 4 | GLU | PRO | LEU |
Entity 2, HP1_CSD 81 residues - Formula weight is not available
| 1 | ASN | ASP | ILE | ALA | ARG | GLY | PHE | GLU | ARG | GLY | ||||
| 2 | LEU | GLU | PRO | GLU | LYS | ILE | ILE | GLY | ALA | THR | ||||
| 3 | ASP | SER | CYS | GLY | ASP | LEU | MET | PHE | LEU | MET | ||||
| 4 | LYS | TRP | LYS | ASP | THR | ASP | GLU | ALA | ASP | LEU | ||||
| 5 | VAL | LEU | ALA | LYS | GLU | ALA | ASN | VAL | LYS | CYS | ||||
| 6 | PRO | GLN | ILE | VAL | ILE | ALA | PHE | TYR | GLU | GLU | ||||
| 7 | ARG | LEU | THR | TRP | HIS | ALA | TYR | PRO | GLU | ASP | ||||
| 8 | ALA | GLU | ASN | LYS | GLU | LYS | GLU | THR | ALA | LYS | ||||
| 9 | SER |
Samples:
sample_1: INCENP, [U-13C; U-15N], 0.1 mM; HP1_CSD 0.15 mM; NaCl 150 mM
sample_conditions_1: ionic strength: 150 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
MAGRO - chemical shift assignment
NMR spectrometers:
- Bruker Avance 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts