BMRB Entry 52207
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52207
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Title: SDHAF4 assembly factor of Human Complex II PubMed: 38212624
Deposition date: 2023-11-10 Original release date: 2023-11-17
Authors: Sharma, Pankaj; Maklashina, Elena; Voehler, Markus; Balinthova, Sona; Dvorakova, Sarka; Kraus, Michal; Hadrava Vanova, Katerina; Nahacka, Zuzana; Zobalova, Renata; Boukalova, Stepana; Cunatova, Kristyna; Mracek, Tomas; Ghayee, Hans; Pacak, Karel; Rohlena, Jakub; Neuzil, Jiri; Cecchini, Gary; Iverson, Tina
Citation: Sharma, Pankaj; Maklashina, Elena; Voehler, Markus; Balinthova, Sona; Dvorakova, Sarka; Kraus, Michal; Hadrava Vanova, Katerina; Nahacka, Zuzana; Zobalova, Renata; Boukalova, Stepana; Cunatova, Kristyna; Mracek, Tomas; Ghayee, Hans; Pacak, Karel; Rohlena, Jakub; Neuzil, Jiri; Cecchini, Gary; Iverson, Tina. "Disordered-to-ordered transitions in assembly factors allow the complex II catalytic subunit to switch binding partners" Nat. Commun. 15, 473-473 (2024).
Assembly members:
entity_1, polymer, 98 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pQT-SDHAF4
Entity Sequences (FASTA):
entity_1: MRSHHHHHHENLYFQGIDPF
TGSSSSQGGKSELVKQSLKK
PKLPEGRFDAPEDSHLEKEP
LEKFPDDVNPVTKEKGGPRG
PEPTRYGDWERKGRCIDF
| Data type | Count |
| 13C chemical shifts | 262 |
| 15N chemical shifts | 93 |
| 1H chemical shifts | 77 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | SDHAF4 | 1 |
Entities:
Entity 1, SDHAF4 98 residues - Formula weight is not available
| 1 | MET | ARG | SER | HIS | HIS | HIS | HIS | HIS | HIS | GLU | ||||
| 2 | ASN | LEU | TYR | PHE | GLN | GLY | ILE | ASP | PRO | PHE | ||||
| 3 | THR | GLY | SER | SER | SER | SER | GLN | GLY | GLY | LYS | ||||
| 4 | SER | GLU | LEU | VAL | LYS | GLN | SER | LEU | LYS | LYS | ||||
| 5 | PRO | LYS | LEU | PRO | GLU | GLY | ARG | PHE | ASP | ALA | ||||
| 6 | PRO | GLU | ASP | SER | HIS | LEU | GLU | LYS | GLU | PRO | ||||
| 7 | LEU | GLU | LYS | PHE | PRO | ASP | ASP | VAL | ASN | PRO | ||||
| 8 | VAL | THR | LYS | GLU | LYS | GLY | GLY | PRO | ARG | GLY | ||||
| 9 | PRO | GLU | PRO | THR | ARG | TYR | GLY | ASP | TRP | GLU | ||||
| 10 | ARG | LYS | GLY | ARG | CYS | ILE | ASP | PHE |
Samples:
sample_1: SDHAF4, [U-100% 13C; U-100% 15N], 9.5 mg/mL; potassium phosphate 20 mM; TCEP 10 mM
sample_conditions_1: ionic strength: 0.025 M; pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D 13C-detected hCONCO | sample_1 | isotropic | sample_conditions_1 |
| 3D 13C-detected hNNCO | sample_1 | isotropic | sample_conditions_1 |
| 2D 13C-detected CON | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN v3.6.2 - collection
NMRViewJ v9.2.0-b24 - chemical shift assignment
TALOS+ v3.80F1 Rev 2012.080.14.41 - data analysis
NMR spectrometers:
- Bruker AVANCE III 900 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts