BMRB Entry 52228

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52228

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Title:
Assignment of trastuzumab-scFab
Deposition date:
2023-12-05
Original release date:
2024-05-02
Authors:
Gagne, Donald; Aramini, James; Aubin, Yves
Citation:

Citation: Gagne, Donald; Aramini, James; Aubin, Yves. "Backbone and methyl side-chain resonance assignments of the single chain Fab fragment of trastuzumab"  Biomol. NMR Assign. 18, 119-128 (2024).
PubMed: 38717571

Assembly members:

Assembly members:
entity_1, polymer, 485 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET15

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GSEVQLVESGGGLVQPGGSL RLSCAASGFNIKDTYIHWVR QAPGKGLEWVARIYPTNGYT RYADSVKGRFTISADTSKNT AYLQMNSLRAEDTAVYYCSR WGGDGFYAMDYWGQGTLVTV SSASTKGPSVFPLAPSSKST SGGTAALGCLVKDYFPEPVT VSWNSGALTSGVHTFPAVLQ SSGLYSLSSVVTVPSSSLGT QTYICNVNHKPSNTKVDKKV EPKSCDKTHTAPGGGGSGGG GSGGGGSGGGGSGGGGSGGG GSSSGLVPRGSDIQMTQSPS SLSASVGDRVTITCRASQDV NTAVAWYQQKPGKAPKLLIY SASFLYSGVPSRFSGSRSGT DFTLTISSLQPEDFATYYCQ QHYTTPPTFGQGTKVEIKRT VAAPSVFIFPPSDEQLKSGT ASVVCLLNNFYPREAKVQWK VDNALQSGNSQESVTEQDSK DSTYSLSSTLTLSKADYEKH KVYACEVTHQGLSSPVTKSF NRGEC

Data sets:
Data typeCount
13C chemical shifts1373
15N chemical shifts405
1H chemical shifts829

Additional metadata:

  • Assembly
  • Samples and Experiments
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Assembly:

Entity Assembly IDEntity NameEntity ID
1trastuzumab-scFab1

Entities:

Entity 1, trastuzumab-scFab 485 residues - Formula weight is not available

1   GLYSERGLUVALGLNLEUVALGLUSERGLY
2   GLYGLYLEUVALGLNPROGLYGLYSERLEU
3   ARGLEUSERCYSALAALASERGLYPHEASN
4   ILELYSASPTHRTYRILEHISTRPVALARG
5   GLNALAPROGLYLYSGLYLEUGLUTRPVAL
6   ALAARGILETYRPROTHRASNGLYTYRTHR
7   ARGTYRALAASPSERVALLYSGLYARGPHE
8   THRILESERALAASPTHRSERLYSASNTHR
9   ALATYRLEUGLNMETASNSERLEUARGALA
10   GLUASPTHRALAVALTYRTYRCYSSERARG
11   TRPGLYGLYASPGLYPHETYRALAMETASP
12   TYRTRPGLYGLNGLYTHRLEUVALTHRVAL
13   SERSERALASERTHRLYSGLYPROSERVAL
14   PHEPROLEUALAPROSERSERLYSSERTHR
15   SERGLYGLYTHRALAALALEUGLYCYSLEU
16   VALLYSASPTYRPHEPROGLUPROVALTHR
17   VALSERTRPASNSERGLYALALEUTHRSER
18   GLYVALHISTHRPHEPROALAVALLEUGLN
19   SERSERGLYLEUTYRSERLEUSERSERVAL
20   VALTHRVALPROSERSERSERLEUGLYTHR
21   GLNTHRTYRILECYSASNVALASNHISLYS
22   PROSERASNTHRLYSVALASPLYSLYSVAL
23   GLUPROLYSSERCYSASPLYSTHRHISTHR
24   ALAPROGLYGLYGLYGLYSERGLYGLYGLY
25   GLYSERGLYGLYGLYGLYSERGLYGLYGLY
26   GLYSERGLYGLYGLYGLYSERGLYGLYGLY
27   GLYSERSERSERGLYLEUVALPROARGGLY
28   SERASPILEGLNMETTHRGLNSERPROSER
29   SERLEUSERALASERVALGLYASPARGVAL
30   THRILETHRCYSARGALASERGLNASPVAL
31   ASNTHRALAVALALATRPTYRGLNGLNLYS
32   PROGLYLYSALAPROLYSLEULEUILETYR
33   SERALASERPHELEUTYRSERGLYVALPRO
34   SERARGPHESERGLYSERARGSERGLYTHR
35   ASPPHETHRLEUTHRILESERSERLEUGLN
36   PROGLUASPPHEALATHRTYRTYRCYSGLN
37   GLNHISTYRTHRTHRPROPROTHRPHEGLY
38   GLNGLYTHRLYSVALGLUILELYSARGTHR
39   VALALAALAPROSERVALPHEILEPHEPRO
40   PROSERASPGLUGLNLEULYSSERGLYTHR
41   ALASERVALVALCYSLEULEUASNASNPHE
42   TYRPROARGGLUALALYSVALGLNTRPLYS
43   VALASPASNALALEUGLNSERGLYASNSER
44   GLNGLUSERVALTHRGLUGLNASPSERLYS
45   ASPSERTHRTYRSERLEUSERSERTHRLEU
46   THRLEUSERLYSALAASPTYRGLULYSHIS
47   LYSVALTYRALACYSGLUVALTHRHISGLN
48   GLYLEUSERSERPROVALTHRLYSSERPHE
49   ASNARGGLYGLUCYS

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks