BMRB Entry 52230

Name: Human Znf706 complexed with cMyc DNA G4Quadruplex heteronuclear NOEs
Published: 2024-08-28

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52230

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Human Znf706 complexed with cMyc DNA G4Quadruplex heteronuclear NOEs

Deposition date:

2023-12-05

Original release date:

2024-08-28

Authors:

Sahoo, Bikash; Bardwell, James

Citation:

Citation: Sahoo, Bikash; Kocman, Vojc; Clark, Nathan; Myers, Nikhil; Deng, Xiexiong; Wong, Ee; Yang, Harry; Kotar, Anita; Guzman, Bryan; Dominguez, Daniel; Plavec, Janez; Bardwell, James. "Protein G-quadruplex interactions and their effects on phase transitions and protein aggregation" Nucleic Acids Res. 52, 4702-4722 (2024).
PubMed: 38572746

Assembly members:

Assembly members:
entity_1, polymer, 76 residues, Formula weight is not available
entity_2, polymer, 19 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MARGQQKIQSQQKNAKKQAG QKKKQGHDQKAAAKAALIYT CTVCRTQMPDPKTFKQHFES KHPKTPLPPELADVQA
entity_2: TAGGGAGGGTAGGGAGGGT

Data sets:

heteronucl_NOEs
- heteronucl_NOEs_1
- heteronucl_NOEs_2

Data type

Count

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Znf706	1
2	cMyc DNA G4 Quadruplex	2

Entities:

Entity 1, Znf706 76 residues - Formula weight is not available

1

MET

ALA

ARG

GLY

GLN

LYS

ILE

GLN

SER

2

GLN

LYS

ASN

ALA

LYS

GLN

ALA

GLY

3

GLN

LYS

GLN

GLY

HIS

ASP

GLN

LYS

4

ALA

LYS

ALA

LEU

ILE

TYR

THR

5

CYS

THR

VAL

CYS

ARG

THR

GLN

MET

PRO

ASP

6

PRO

LYS

THR

PHE

LYS

GLN

HIS

PHE

GLU

SER

7

LYS

HIS

PRO

LYS

THR

PRO

LEU

PRO

GLU

8

LEU

ALA

ASP

VAL

GLN

ALA

Entity 2, cMyc DNA G4 Quadruplex 19 residues - Formula weight is not available

1

DT

DA

DG

DA

DG

DT

2

DA

DG

DA

DG

DT

Samples:

sample_1: Human Znf706, [U-100% 15N], 0.2 mM; cMyc DNA G4 quadruplex 0.1 mM; Sodium phosphate buffer 20 mM; KCl 100 mM

sample_conditions_1: ionic strength: 0.1 M; pH: 7.4; pressure: 1 atm; temperature: 277.1 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H-15N heteronoe	sample_1	isotropic	sample_conditions_1

Software:

NMRFAM-SPARKY - data analysis

NMR spectrometers:

Bruker Avance 600 MHz