BMRB Entry 5224
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5224
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Title: 1H, 13C and 15N Chemical shifts assignments for the C-terminal domain (region 428-547) of human lamin A/C PubMed: 12018485
Deposition date: 2001-12-05 Original release date: 2002-08-22
Authors: Krimm, Isabelle; Couprie, Joel; Ostlund, Cecilia; Worman, Howard; Zinn-Justin, Sophie
Citation: Krimm, Isabelle; Couprie, Joel; Ostlund, Cecilia; Worman, Howard; Zinn-Justin, Sophie. "Letter to the Editor: 1H, 13C and 15N resonance assignments of the C-terminal domain of human lamin A/C " J. Biomol. NMR 22, 371-372 (2002).
Assembly members:
lamin A/C, polymer, 120 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
lamin A/C: SSFSQHARTSGRVAVEEVDE
EGKFVRLRNKSNEDQSMGNW
QIKRQNGDDPLLTYRFPPKF
TLKAGQVVTIWAAGAGATHS
PPTDLVWKAQNTWGCGNSLR
TALINSTGEEVAMRKLVRSV
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 802 |
| 13C chemical shifts | 492 |
| 15N chemical shifts | 127 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | lamin | 1 |
Entities:
Entity 1, lamin 120 residues - Formula weight is not available
| 1 | SER | SER | PHE | SER | GLN | HIS | ALA | ARG | THR | SER | |
| 2 | GLY | ARG | VAL | ALA | VAL | GLU | GLU | VAL | ASP | GLU | |
| 3 | GLU | GLY | LYS | PHE | VAL | ARG | LEU | ARG | ASN | LYS | |
| 4 | SER | ASN | GLU | ASP | GLN | SER | MET | GLY | ASN | TRP | |
| 5 | GLN | ILE | LYS | ARG | GLN | ASN | GLY | ASP | ASP | PRO | |
| 6 | LEU | LEU | THR | TYR | ARG | PHE | PRO | PRO | LYS | PHE | |
| 7 | THR | LEU | LYS | ALA | GLY | GLN | VAL | VAL | THR | ILE | |
| 8 | TRP | ALA | ALA | GLY | ALA | GLY | ALA | THR | HIS | SER | |
| 9 | PRO | PRO | THR | ASP | LEU | VAL | TRP | LYS | ALA | GLN | |
| 10 | ASN | THR | TRP | GLY | CYS | GLY | ASN | SER | LEU | ARG | |
| 11 | THR | ALA | LEU | ILE | ASN | SER | THR | GLY | GLU | GLU | |
| 12 | VAL | ALA | MET | ARG | LYS | LEU | VAL | ARG | SER | VAL |
Samples:
sample_1: lamin A/C, [U-13C; U-15N], 0.5 – 1.5 mM
Ex-cond_1: pH*: 6.3; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N NOESY | not available | not available | not available |
| HNCA | not available | not available | not available |
| HNCOCA | not available | not available | not available |
| HNCO | not available | not available | not available |
| HNCACB | not available | not available | not available |
| CBCACONH | not available | not available | not available |
| HCCH-TOCSY | not available | not available | not available |
| 1H-13C NOESY | not available | not available | not available |
Software:
NMR-Pipe -
Felix -
NMR spectrometers:
- Bruker DRX 500 MHz
- Bruker DRX 600 MHz
- Bruker DRX 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAD51965 BAE72960 BAE87519 BAF62394 BAG57524 |
| EMBL | CAA27173 CAA27174 CAJ28141 |
| GB | AAA36160 AAA36164 AAH00511 AAH03162 AAH14507 |
| PRF | 1203377A |
| REF | NP_001029225 NP_001092024 NP_001104727 NP_001244303 NP_001244822 |
| SP | P02545 Q3ZD69 |
| TPG | DAA31761 |
| AlphaFold | Q3ZD69 P02545 |
Download HSQC peak lists in one of the following formats:
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