BMRB Entry 52243

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52243

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Title:
Enabling Site-Specific NMR Investigations of Therapeutic Fab using a Cell-Free Based Isotopic Labeling Approach: Application to anti-LAMP1 Fab.
Deposition date:
2023-12-14
Original release date:
2024-08-28
Authors:
Giraud, Arthur; Imbert, Lionel; Favier, Adrien; Henot, Faustine; Duffieux, Francis; Samson, Camille; Frances, Oriane; Crublet, Elodie; Boisbouvier, Jerome
Citation:

Citation: Giraud, Arthur; Imbert, Lionel; Favier, Adrien; Henot, Faustine; Duffieux, Francis; Samson, Camille; Samson, Oriane; Crublet, Elodie; Boisbouvier, Jerome. "Enabling site-specific NMR investigations of therapeutic Fab using a cell-free based isotopic labeling approach: application to anti-LAMP1 Fab"  J. Biomol. NMR 78, 73-86 (2024).
PubMed: 38546905

Assembly members:

Assembly members:
entity_1, polymer, 214 residues, Formula weight is not available
entity_2, polymer, 227 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis   Host organism: Escherichia coli   Vector: pIVEX 2.4d

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GDIQMTQSPSSLSASVGDRV TITCKASQDIDRYMAWYQDK PGKAPRLLIHDTSTLQSGVP SRFSGSGSGRDYTLTISNLE PEDFATYYCLQYDNLWTFGG GTKVEIKRTVAAPSVFIFPP SDEQLKSGTASVVCLLNNFY PREAKVQWKVDNALQSGNSQ ESVTEQDSKDSTYSLSSTLT LSKADYEKHKVYACEVTHQG LSSPVTKSFNRGEC
entity_2: GQVQLVQSGAEVKKPGSSVK VSCKASGYIFTNYNIHWVKK SPGQGLEWIGAIYPGNGDAP YSQKFQGKATLTADTSTSTT YMELSSLRSEDTAVYYCVRA NWDVAFAYWGQGTLVTVSSA STKGPSVFPLAPSSKSTSGG TAALGCLVKDYFPEPVTVSW NSGALTSGVHTFPAVLQSSG LYSLSSVVTVPSSSLGTQTY ICNVNHKPSNTKVDKKVEPK SCDKTHT

Data sets:
Data typeCount
13C chemical shifts1176
15N chemical shifts372
1H chemical shifts372

Additional metadata:

  • Assembly
  • Samples and Experiments
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  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Anti-LAMP1 Fab fragment light chain1
2Anti-LAMP1 Fab fragment heavy chain2

Entities:

Entity 1, Anti-LAMP1 Fab fragment light chain 214 residues - Formula weight is not available

The sequence of the Fab fragment is composed with two amino acids chains: the light chain and the heavy chain. The two chain are linked thanks to a disulfide bond between two cysteines at their C-terminal.

1   GLYASPILEGLNMETTHRGLNSERPROSER
2   SERLEUSERALASERVALGLYASPARGVAL
3   THRILETHRCYSLYSALASERGLNASPILE
4   ASPARGTYRMETALATRPTYRGLNASPLYS
5   PROGLYLYSALAPROARGLEULEUILEHIS
6   ASPTHRSERTHRLEUGLNSERGLYVALPRO
7   SERARGPHESERGLYSERGLYSERGLYARG
8   ASPTYRTHRLEUTHRILESERASNLEUGLU
9   PROGLUASPPHEALATHRTYRTYRCYSLEU
10   GLNTYRASPASNLEUTRPTHRPHEGLYGLY
11   GLYTHRLYSVALGLUILELYSARGTHRVAL
12   ALAALAPROSERVALPHEILEPHEPROPRO
13   SERASPGLUGLNLEULYSSERGLYTHRALA
14   SERVALVALCYSLEULEUASNASNPHETYR
15   PROARGGLUALALYSVALGLNTRPLYSVAL
16   ASPASNALALEUGLNSERGLYASNSERGLN
17   GLUSERVALTHRGLUGLNASPSERLYSASP
18   SERTHRTYRSERLEUSERSERTHRLEUTHR
19   LEUSERLYSALAASPTYRGLULYSHISLYS
20   VALTYRALACYSGLUVALTHRHISGLNGLY
21   LEUSERSERPROVALTHRLYSSERPHEASN
22   ARGGLYGLUCYS

Entity 2, Anti-LAMP1 Fab fragment heavy chain 227 residues - Formula weight is not available

The sequence of the Fab fragment is composed with two amino acids chains: the light chain and the heavy chain. The two chain are linked thanks to a disulfide bond between two cysteines at their C-terminal.

1   GLYGLNVALGLNLEUVALGLNSERGLYALA
2   GLUVALLYSLYSPROGLYSERSERVALLYS
3   VALSERCYSLYSALASERGLYTYRILEPHE
4   THRASNTYRASNILEHISTRPVALLYSLYS
5   SERPROGLYGLNGLYLEUGLUTRPILEGLY
6   ALAILETYRPROGLYASNGLYASPALAPRO
7   TYRSERGLNLYSPHEGLNGLYLYSALATHR
8   LEUTHRALAASPTHRSERTHRSERTHRTHR
9   TYRMETGLULEUSERSERLEUARGSERGLU
10   ASPTHRALAVALTYRTYRCYSVALARGALA
11   ASNTRPASPVALALAPHEALATYRTRPGLY
12   GLNGLYTHRLEUVALTHRVALSERSERALA
13   SERTHRLYSGLYPROSERVALPHEPROLEU
14   ALAPROSERSERLYSSERTHRSERGLYGLY
15   THRALAALALEUGLYCYSLEUVALLYSASP
16   TYRPHEPROGLUPROVALTHRVALSERTRP
17   ASNSERGLYALALEUTHRSERGLYVALHIS
18   THRPHEPROALAVALLEUGLNSERSERGLY
19   LEUTYRSERLEUSERSERVALVALTHRVAL
20   PROSERSERSERLEUGLYTHRGLNTHRTYR
21   ILECYSASNVALASNHISLYSPROSERASN
22   THRLYSVALASPLYSLYSVALGLUPROLYS
23   SERCYSASPLYSTHRHISTHR

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks