BMRB Entry 52255

Name: Mdm2aa211-223 pS215-pT218
Published: 2024-01-08

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52255

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Mdm2aa211-223 pS215-pT218

Deposition date: 2024-01-02 Original release date: 2024-01-08

Authors: Luo, Yingyue; Theillet, Francois-Xavier

Citation: Luo, Yingyue; Theillet, Francois-Xavier. "Structural characterization of the MDM2 NLS/NES/arrestin-binding/acidic domain in phospho- and unmodified-forms" .

Assembly members:
entity_1, polymer, 15 residues, Formula weight is not available

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source: Production method: chemical synthesis

Entity Sequences (FASTA):
entity_1: XSSSEXTGXPSNPDX

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
15N chemical shifts	11
1H chemical shifts	49

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Mdm2aa211-223_pS215-pT218	1

Entities:

Entity 1, Mdm2aa211-223_pS215-pT218 15 residues - Formula weight is not available

Ac-SSSEpSTGpTPSNPD-NH2

1

ACE

SER

GLU

SEP

THR

GLY

TPO

PRO

2

SER

ASN

PRO

ASP

NH2

Samples:

sample_1: Mdm2aa211_223_pS215_pT218 3 mM; sodium phosphate 20 mM; sodium chloride 150 mM; DSS 0.5 mM

sample_conditions_1: ionic strength: 0.190 M; pH: 7.1; pressure: 1 atm; temperature: 283 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H ROESY	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN v4 - collection

TOPSPIN v3 - collection

CcpNMR v3 - chemical shift assignment

NMR spectrometers:

Bruker AVANCE NEO 700 MHz
Bruker AVANCE III 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts