BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules 		Member of WWPDB

BMRB Entry 52258

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52258

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Title: Efg1 N prion-like domain assignments   PubMed: 38297837

Deposition date: 2024-01-03 Original release date: 2024-02-13

Authors: Zheng, Tongyin; Wang, Szu-Huan; Fawzi, Nicolas

Citation: Wang, Szu-Huan; Zheng, Tongyin; Fawzi, Nicolas. "Structure and interactions of prion-like domains in transcription factor Efg1 phase separation"  Biophys. J. 123, 1481-1493 (2024).

Assembly members:
entity_1, polymer, 181 residues, Formula weight is not available

Natural source:   Common Name: budding yeasts   Taxonomy ID: 5476   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Candida albicans

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pTHMT

Entity Sequences (FASTA):
entity_1: MSTYSIPYYNQMNGNYNNGM PQQTTAANQQAFPQQQQPTT TGNASQQQQQAAATAAAAAV QQPYNYMFYQQQGQPGQQTG QTAGQQQQQQQQQQQYDYNT YNRYQYPAATSQGNYYQQTI PNQLSQPQPQHYNGSNRNYT SAPSGAPIPSNSTSGPSQQP PLPGQQAVPIPPHVSTMQQP T

Data sets:
Data typeCount
13C chemical shifts510
15N chemical shifts154
1H chemical shifts154

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Efg1 PrLD1

Entities:

Entity 1, Efg1 PrLD 181 residues - Formula weight is not available

1   METSERTHRTYRSERILEPROTYRTYRASN
2   GLNMETASNGLYASNTYRASNASNGLYMET
3   PROGLNGLNTHRTHRALAALAASNGLNGLN
4   ALAPHEPROGLNGLNGLNGLNPROTHRTHR
5   THRGLYASNALASERGLNGLNGLNGLNGLN
6   ALAALAALATHRALAALAALAALAALAVAL
7   GLNGLNPROTYRASNTYRMETPHETYRGLN
8   GLNGLNGLYGLNPROGLYGLNGLNTHRGLY
9   GLNTHRALAGLYGLNGLNGLNGLNGLNGLN
10   GLNGLNGLNGLNGLNTYRASPTYRASNTHR
11   TYRASNARGTYRGLNTYRPROALAALATHR
12   SERGLNGLYASNTYRTYRGLNGLNTHRILE
13   PROASNGLNLEUSERGLNPROGLNPROGLN
14   HISTYRASNGLYSERASNARGASNTYRTHR
15   SERALAPROSERGLYALAPROILEPROSER
16   ASNSERTHRSERGLYPROSERGLNGLNPRO
17   PROLEUPROGLYGLNGLNALAVALPROILE
18   PROPROHISVALSERTHRMETGLNGLNPRO
19   THR

Samples:

sample_1: Efg1 N-PrLD, [U-100% 13C; U-100% 15N], 100 uM; MES 20 mM

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCACONHsample_1isotropicsample_conditions_1

Software:

TOPSPIN - collection

CcpNMR - data analysis

NMRPipe - processing

NMR spectrometers:

  • Bruker Avance 850 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts