BMRB Entry 52277

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52277

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Title:
Backbone resonances of C-Mad2 R133A bound to Cdc20-MIM
Deposition date:
2024-01-23
Original release date:
2025-05-19
Authors:
Yu, Conny; Fischer, Elyse; Greener, Joe; Freund, Stefan; Barford, David
Citation:

Citation: Yu, Conny; Fischer, Elyse; Greener, Joe; Yang, Jing; Zhang, Ziguo; Freund, Stefan; Barford, David. "Molecular mechanism of Mad2 conformational conversion promoted by the Mad2-interaction motif of Cdc20"  Protein Sci. 34, e70099-e70099 (2025).
PubMed: 40143766

Assembly members:

Assembly members:
entity_1, polymer, 205 residues, Formula weight is not available
entity_2, polymer, 28 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MALQLSREQGITARGSAEIV AEFFSFGINSILYQRGIYPS ETFTRVQKYGLTLLVTTDLE LIKYLNNVVEQLKDWLYKCS VQKLVVVISNIESGEVLERW QFDIECDKTAKDDSAPREKS QKAIQDEIRSVIAQITATVT FLPLLEVSCSFDLLIYTDKD LVVPEKWEESGPQFITNSEE VRLRSFTTTIHKVNSMVAYK IPVND
entity_2: EHQKAWALNLNGFDVEEAKI LRLSGKPQ

Data sets:
Data typeCount
13C chemical shifts574
15N chemical shifts185
1H chemical shifts184

Additional metadata:

  • Assembly
  • Samples and Experiments
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Assembly:

Entity Assembly IDEntity NameEntity ID
1O-Mad21
2Cdc20 MIM2

Entities:

Entity 1, O-Mad2 205 residues - Formula weight is not available

1   METALALEUGLNLEUSERARGGLUGLNGLY
2   ILETHRALAARGGLYSERALAGLUILEVAL
3   ALAGLUPHEPHESERPHEGLYILEASNSER
4   ILELEUTYRGLNARGGLYILETYRPROSER
5   GLUTHRPHETHRARGVALGLNLYSTYRGLY
6   LEUTHRLEULEUVALTHRTHRASPLEUGLU
7   LEUILELYSTYRLEUASNASNVALVALGLU
8   GLNLEULYSASPTRPLEUTYRLYSCYSSER
9   VALGLNLYSLEUVALVALVALILESERASN
10   ILEGLUSERGLYGLUVALLEUGLUARGTRP
11   GLNPHEASPILEGLUCYSASPLYSTHRALA
12   LYSASPASPSERALAPROARGGLULYSSER
13   GLNLYSALAILEGLNASPGLUILEARGSER
14   VALILEALAGLNILETHRALATHRVALTHR
15   PHELEUPROLEULEUGLUVALSERCYSSER
16   PHEASPLEULEUILETYRTHRASPLYSASP
17   LEUVALVALPROGLULYSTRPGLUGLUSER
18   GLYPROGLNPHEILETHRASNSERGLUGLU
19   VALARGLEUARGSERPHETHRTHRTHRILE
20   HISLYSVALASNSERMETVALALATYRLYS
21   ILEPROVALASNASP

Entity 2, Cdc20 MIM 28 residues - Formula weight is not available

1   GLUHISGLNLYSALATRPALALEUASNLEU
2   ASNGLYPHEASPVALGLUGLUALALYSILE
3   LEUARGLEUSERGLYLYSPROGLN

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks