BMRB Entry 52286
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52286
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Title: Backbone Assignments of Ydj1 J-domain and Gly-rich region PubMed: 38529663
Deposition date: 2024-01-25 Original release date: 2024-04-12
Authors: Ciesielski, Szymon; Tonelli, Marco; Craig, Elizabeth
Citation: Ciesielski, Szymon; Schilke, Brenda; Stolarska, Milena; Tonelli, Marco; Tomiczek, Bartlomiej; Craig, Elizabeth. "Comparative structural and functional analysis of the glycine-rich regions of Class A and B J-domain protein cochaperones of Hsp70" FEBS Lett. ., .-. (2024).
Assembly members:
entity_1, polymer, 114 residues, Formula weight is not available
Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pSmt3-ydj1109
Entity Sequences (FASTA):
entity_1: GEFGSMVKETKFYDILGVPV
TATDVEIKKAYRKCALKYHP
DKNPSEEAAEKFKEASAAYE
ILSDPEKRDIYDQFGEDGLS
GAGGAGGFPGGGFGFGDDIF
SQFFGAGGAQRPRG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 205 |
| 15N chemical shifts | 105 |
| 1H chemical shifts | 105 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Ydj1 J-domain and Gly-rich region (1-109 residues) | 1 |
Entities:
Entity 1, Ydj1 J-domain and Gly-rich region (1-109 residues) 114 residues - Formula weight is not available
| 1 | GLY | GLU | PHE | GLY | SER | MET | VAL | LYS | GLU | THR | ||||
| 2 | LYS | PHE | TYR | ASP | ILE | LEU | GLY | VAL | PRO | VAL | ||||
| 3 | THR | ALA | THR | ASP | VAL | GLU | ILE | LYS | LYS | ALA | ||||
| 4 | TYR | ARG | LYS | CYS | ALA | LEU | LYS | TYR | HIS | PRO | ||||
| 5 | ASP | LYS | ASN | PRO | SER | GLU | GLU | ALA | ALA | GLU | ||||
| 6 | LYS | PHE | LYS | GLU | ALA | SER | ALA | ALA | TYR | GLU | ||||
| 7 | ILE | LEU | SER | ASP | PRO | GLU | LYS | ARG | ASP | ILE | ||||
| 8 | TYR | ASP | GLN | PHE | GLY | GLU | ASP | GLY | LEU | SER | ||||
| 9 | GLY | ALA | GLY | GLY | ALA | GLY | GLY | PHE | PRO | GLY | ||||
| 10 | GLY | GLY | PHE | GLY | PHE | GLY | ASP | ASP | ILE | PHE | ||||
| 11 | SER | GLN | PHE | PHE | GLY | ALA | GLY | GLY | ALA | GLN | ||||
| 12 | ARG | PRO | ARG | GLY |
Samples:
sample_1: Ydj1 1-109, [U-100% 13C; U-100% 15N], 300 uM; TRIS 20 mM; sodium chloride 150 mM; DTT 5 mM; sodium azide 0.02%
sample_conditions_1: ionic strength: 300 mM; pH: 7.5; pressure: 1 atm; temperature: 278 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN - collection
VNMRj - collection
NMRPipe - processing
SMILE - processing
NMRFAM-SPARKY - data analysis
PINE - data analysis
APES - data analysis
NMR spectrometers:
- Bruker AVANCE III 900 MHz
- Varian VXRS 800 MHz
- Bruker AVANCE III 750 MHz
- Bruker AVANCE III 600 MHz
- Varian VXRS 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts