BMRB Entry 52323

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52323

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Title:
An extended interaction site determines binding between AP180 and AP2 in clathrin mediated endocytosis
Deposition date:
2024-02-16
Original release date:
2024-06-25
Authors:
Naudi-Fabra, Samuel; Elena-Real, Carlos; Vedel, Ida; Tengo, Maud; Motzny, Kathrin; Jiang, Pin-Lian; Schmieder, Peter; Liu, Fan; Milles, Sigrid
Citation:

Citation: Naudi-Fabra, Samuel; Elena-Real, Carlos; Vedel, Ida; Tengo, Maud; Motzny, Kathrin; Jiang, Pin-Lian; Schmieder, Peter; Liu, Fan; Milles, Sigrid. "An extended interaction site determines binding between AP180 and AP2 in clathrin mediated endocytosis"  Nat. Commun. 15, 5884-5884 (2024).
PubMed: 39003270

Assembly members:

Assembly members:
entity_1, polymer, 201 residues, 18977.69 Da.

Natural source:

Natural source:   Common Name: Rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-28

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: SDPFAPEPSPPTTTTEPASA SASATTAVTAATTEVDLFGD AFAASPGEAPAASEGATAPA TPAPVAAALDACSGNDPFAP SEGSAEAAPELDLFAMKPPE TSAPVVTPTASTAPPVPATA PSPAPTAVAATAAATTTTTA AAATTTATTSAAAAATAAAP PALDIFGDLFDSAPEVAAAS KPDVAPSIDLFGTDAFSSPP R

Data sets:
Data typeCount
13C chemical shifts415
15N chemical shifts142
1H chemical shifts143

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1AP180 399-5981

Entities:

Entity 1, AP180 399-598 201 residues - 18977.69 Da.

1   SERASPPROPHEALAPROGLUPROSERPRO
2   PROTHRTHRTHRTHRGLUPROALASERALA
3   SERALASERALATHRTHRALAVALTHRALA
4   ALATHRTHRGLUVALASPLEUPHEGLYASP
5   ALAPHEALAALASERPROGLYGLUALAPRO
6   ALAALASERGLUGLYALATHRALAPROALA
7   THRPROALAPROVALALAALAALALEUASP
8   ALACYSSERGLYASNASPPROPHEALAPRO
9   SERGLUGLYSERALAGLUALAALAPROGLU
10   LEUASPLEUPHEALAMETLYSPROPROGLU
11   THRSERALAPROVALVALTHRPROTHRALA
12   SERTHRALAPROPROVALPROALATHRALA
13   PROSERPROALAPROTHRALAVALALAALA
14   THRALAALAALATHRTHRTHRTHRTHRALA
15   ALAALAALATHRTHRTHRALATHRTHRSER
16   ALAALAALAALAALATHRALAALAALAPRO
17   PROALALEUASPILEPHEGLYASPLEUPHE
18   ASPSERALAPROGLUVALALAALAALASER
19   LYSPROASPVALALAPROSERILEASPLEU
20   PHEGLYTHRASPALAPHESERSERPROPRO
21   ARG

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