BMRB Entry 5236

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PDB ID: 1kn7
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5236
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H and 15N Chemical Shift Assignments for the tandem inactivation domain of Kv1.4 (RCK4(1-75))

Deposition date:

2001-12-18

Original release date:

2003-04-30

Authors:

Wissmann, Ralph; Bildl, Wolfgang; Oliver, Dominik; Jonas, Peter; Bentrop, Detlef; Fakler, Bernd

Citation:

Citation: Wissmann, Ralph; Bildl, Wolfgang; Oliver, Dominik; Beyermann, M.; Kalbitzer, H.; Bentrop, Detlef; Fakler, Bernd. "Solution Structure and Function of the "Tandem Inactivation Domain" of the Neuronal A-type Potassium Channel Kv1.4." J. Biol. Chem. 278, 16142-16150 (2003).
PubMed: 12590144

Assembly members:

Assembly members:
RCK4, polymer, 78 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Norway rat Taxonomy ID: 10116 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rattus norvegicus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET41a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RCK4: GSTMEVAMVSAESSGCNSHM PYGYAAQARARERERLAHSR AAAAAAVAAATAAVEGTGGS GGGPHHHHQTRGAYSSHD

Data sets:

assigned_chemical_shifts
- shift_set_1
coupling_constants
- J_values_set_1

Data type	Count
1H chemical shifts	397
15N chemical shifts	76
coupling constants	62

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	RCK4(1-75)	1

Entities:

Entity 1, RCK4(1-75) 78 residues - Formula weight is not available

1

GLY

SER

THR

MET

GLU

VAL

ALA

MET

VAL

SER

2

ALA

GLU

SER

GLY

CYS

ASN

SER

HIS

MET

3

PRO

TYR

GLY

TYR

ALA

GLN

ALA

ARG

ALA

4

ARG

GLU

ARG

GLU

ARG

LEU

ALA

HIS

SER

ARG

5

ALA

VAL

ALA

6

THR

ALA

VAL

GLU

GLY

THR

GLY

SER

7

GLY

PRO

HIS

GLN

THR

8

ARG

GLY

ALA

TYR

SER

HIS

ASP

Samples:

sample_1: RCK4, [U-98% 15N], 0.8 – 1.0 mM; sodium phosphate 50 mM; DTT 10 mM; pefablock SC 1 mM

Ex-cond_1: pH: 4.4; temperature: 283 K; ionic strength: 0.05 M

Experiments:

Name	Sample	Sample state	Sample conditions
1H-15N NOESY	not available	not available	not available
1H-15N TOCSY	not available	not available	not available
HNHA	not available	not available	not available
2D NOESY	not available	not available	not available

Software:

No software information available

NMR spectrometers:

Bruker Avance 600 MHz

BMRB	19426
PDB	1KN7
DBJ	BAC29309
EMBL	CAA34133
GB	AAA41469 AAB60668 AAI09015 EDL27774 EDL79747
REF	NP_037103 NP_067250 XP_003497608 XP_005064870 XP_005364106
SP	P15385 Q61423
AlphaFold	P15385 Q61423