BMRB Entry 52384

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PDB ID: 9fdg
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52384
MolProbity Validation Chart

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Title:
Chemical shift assignments of a de novo designed 12 stranded transmembrane beta barrel
Deposition date:
2024-04-02
Original release date:
2024-07-22
Authors:
Muntener, Thomas; Hiller, Sebastian
Citation:

Citation: Berhanu, Samuel; Majumder, Sagardip; Muntener, Thomas; Whitehouse, James; Berner, Carolin; Bera, Asim; Kang, Alex; Liang, Binyong; Khan, G Nasir; Sankaran, Banumathi; Tamm, Lukas; Brockwell, David; Hiller, Sebastian; Radford, Sheena; Baker, David; Vorobieva, Anastassia. "Sculpting conducting nanopore size and shape through de novo protein design"  Science 385, 282-288 (2024).
PubMed: 39024453

Assembly members:

Assembly members:
entity_1, polymer, 179 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET29b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: QDKPGSAKAGGWTTYNTDNT FKGGSYAKYVLSPNLALKGE YEWNNSSLNSFKAGAEYVAT PYLKTEVMTEYNTDNTFRVT VVTEGRYPVDPNLELFPGGW YTWNNSSLNKGAPYTRAEYK LTPDLKLLSQVVYNTDNTFK FDTGLEYKLSPNLKVKFEYG WNNSSLNEFTVQFEYDLSS

Data sets:
Data typeCount
13C chemical shifts194
15N chemical shifts103
1H chemical shifts181

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1TMB1121

Entities:

Entity 1, TMB112 179 residues - Formula weight is not available

1   GLNASPLYSPROGLYSERALALYSALAGLY
2   GLYTRPTHRTHRTYRASNTHRASPASNTHR
3   PHELYSGLYGLYSERTYRALALYSTYRVAL
4   LEUSERPROASNLEUALALEULYSGLYGLU
5   TYRGLUTRPASNASNSERSERLEUASNSER
6   PHELYSALAGLYALAGLUTYRVALALATHR
7   PROTYRLEULYSTHRGLUVALMETTHRGLU
8   TYRASNTHRASPASNTHRPHEARGVALTHR
9   VALVALTHRGLUGLYARGTYRPROVALASP
10   PROASNLEUGLULEUPHEPROGLYGLYTRP
11   TYRTHRTRPASNASNSERSERLEUASNLYS
12   GLYALAPROTYRTHRARGALAGLUTYRLYS
13   LEUTHRPROASPLEULYSLEULEUSERGLN
14   VALVALTYRASNTHRASPASNTHRPHELYS
15   PHEASPTHRGLYLEUGLUTYRLYSLEUSER
16   PROASNLEULYSVALLYSPHEGLUTYRGLY
17   TRPASNASNSERSERLEUASNGLUPHETHR
18   VALGLNPHEGLUTYRASPLEUSERSER

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