BMRB Entry 52414
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52414
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Title: 1H-NMR assignments for the Z0 zinc-finger domain of transcription repressor Bcl11A
Deposition date: 2024-04-16 Original release date: 2024-05-20
Authors: Alexandrescu, Andrei
Citation: Alexandrescu, Andrei. "Solution structure of the Z0 domain from transcription repressor Bcl11A provides clues about protein-binding zinc finger sequences" To be determined ., .-..
Assembly members:
entity_1, polymer, 27 residues, Formula weight is not available
entity_ZN, non-polymer, 65.409 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
entity_1: LTCGQCQMNFPLGDILIFIE
HKRKQCN
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 34 |
| 1H chemical shifts | 193 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Z0 | 1 |
| 2 | ZINC ION | 2 |
Entities:
Entity 1, Z0 27 residues - Formula weight is not available
Corresponds to residues 46-72 of the human BCl11A sequence (UniProt ID: Q9H165)
| 1 | LEU | THR | CYS | GLY | GLN | CYS | GLN | MET | ASN | PHE | ||||
| 2 | PRO | LEU | GLY | ASP | ILE | LEU | ILE | PHE | ILE | GLU | ||||
| 3 | HIS | LYS | ARG | LYS | GLN | CYS | ASN |
Entity 2, ZINC ION - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: Bcl11A-Z0 40 uM; ZINC ION 600 uM
sample_2: Bcl11A-Z0 40 uM; ZINC ION 600 uM
sample_conditions_1: ionic strength: 0.0006 M; pH: 6.3; pressure: 1 atm; temperature: 298 K
sample_conditions_2: ionic strength: 0.0006 M; pH: 5.8; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_2 | isotropic | sample_conditions_2 |
| 2D 1H-1H NOESY | sample_2 | isotropic | sample_conditions_2 |
| 2D 1H-1H NOESY | sample_2 | isotropic | sample_conditions_2 |
| 2D 1H-1H TOCSY | sample_2 | isotropic | sample_conditions_2 |
Software:
VNMRj - collection
CcpNMR v2.5.2 - chemical shift assignment
X-PLOR NIH v3.8 - structure solution
NMR spectrometers:
- Varian INOVA 600 MHz