BMRB Entry 52416

Name: Resonance assignment of a loc1p region
Published: 2024-05-20

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52416

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Resonance assignment of a loc1p region

Deposition date: 2024-04-19 Original release date: 2024-05-20

Authors: Hennig, Janosch; Sattler, Michael

Citation: Niedner-Boblenz, Annika; Monecke, Thomas; Hennig, Janosch; Klostermann, Melina; Hofweber, Mario; Davydenko, Elena; Gerber, Andre; Anosova, Irina; Mayer, Wieland; Muller, Marisa; Heym, Roland; Janowski, Robert; Paillart, Jean-Christophe; Dormann, Dorothee; Zarnack, Kathi; Sattler, Michael; Niessing, Dierk. "Intrinsically disordered RNA-binding motifs cooperate to catalyze RNA folding and drive phase separation" BioRxiv ., .-. (2024).

Assembly members:
entity_1, polymer, 15 residues, Formula weight is not available

Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-M43

Entity Sequences (FASTA):
entity_1: GSPKKPSKRQNLRRE

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	53
15N chemical shifts	13
1H chemical shifts	106

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	loc1p	1

Entities:

Entity 1, loc1p 15 residues - Formula weight is not available

1

GLY

SER

PRO

LYS

PRO

SER

LYS

ARG

GLN

2

ASN

LEU

ARG

GLU

Samples:

sample_1: loc1p, [U-99% 13C; U-99% 15N], 1.6 mM; NaPO4 20 mM; NaCl 50 mM

sample_conditions_1: ionic strength: 50 mM; pH: 7.4; pressure: 1 atm; temperature: 278 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D (H)CCH-TOCSY	sample_1	isotropic	sample_conditions_1

Software:

CARA - chemical shift assignment

NMR spectrometers:

Bruker AVANCE III 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts