BMRB Entry 52470

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52470

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Title:
NMR backbone chemical shifts of the SARS-CoV-2 Nucleocapsid N-terminal domain Y109A mutant
Deposition date:
2024-05-18
Original release date:
2024-11-11
Authors:
Dhamotharan, Karthikeyan; Korn, Sophie; Wacker, Anna; Becker, Matthias; Schwalbe, Harald; Schlundt, Andreas
Citation:

Citation: Dhamotharan, Karthikeyan; Wacker, Anna; Becker, Matthias; Gunther, Sebastian; Schwalbe, Harald; Korn, Sophie; Schlundt, Andreas. "Conserved residues in the SARS-CoV-2 nucleocapsid N-terminal domain coordinate structural stability and steer RNA recognition"  .

Assembly members:

Assembly members:
entity_1, polymer, 138 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: SARS-CoV-2   Taxonomy ID: 2697049   Superkingdom: Viruses   Kingdom: not available   Genus/species: Betacoronavirus HCoV-SARS

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-Sumo

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GGLPNNTASWFTALTQHGKE DLKFPRGQGVPINTNSSPDD QIGYYRRATRRIRGGDGKMK DLSPRWAFYYLGTGPEAGLP YGANKDGIIWVATEGALNTP KDHIGTRNPANNAAIVLQLP QGTTLPKGFYAEGSRGGS

Data sets:
Data typeCount
13C chemical shifts391
15N chemical shifts127
1H chemical shifts127

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1NTD Y109A1

Entities:

Entity 1, NTD Y109A 138 residues - Formula weight is not available

The first G is a cloning artefact. The second G is residue 44 of the natural sequence.

1   GLYGLYLEUPROASNASNTHRALASERTRP
2   PHETHRALALEUTHRGLNHISGLYLYSGLU
3   ASPLEULYSPHEPROARGGLYGLNGLYVAL
4   PROILEASNTHRASNSERSERPROASPASP
5   GLNILEGLYTYRTYRARGARGALATHRARG
6   ARGILEARGGLYGLYASPGLYLYSMETLYS
7   ASPLEUSERPROARGTRPALAPHETYRTYR
8   LEUGLYTHRGLYPROGLUALAGLYLEUPRO
9   TYRGLYALAASNLYSASPGLYILEILETRP
10   VALALATHRGLUGLYALALEUASNTHRPRO
11   LYSASPHISILEGLYTHRARGASNPROALA
12   ASNASNALAALAILEVALLEUGLNLEUPRO
13   GLNGLYTHRTHRLEUPROLYSGLYPHETYR
14   ALAGLUGLYSERARGGLYGLYSER

Related Database Links:

UNP P0DTC9

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks