BMRB Entry 52472

Name: NMR backbone chemical shifts of the SARS-CoV-2 Nucleocapsid N-terminal domain P67S mutant
Published: 2024-11-11

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52472

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR backbone chemical shifts of the SARS-CoV-2 Nucleocapsid N-terminal domain P67S mutant

Deposition date:

2024-05-19

Original release date:

2024-11-11

Authors:

Dhamotharan, Karthikeyan; Korn, Sophie; Wacker, Anna; Becker, Matthias; Schwalbe, Harald; Schlundt, Andreas

Citation:

Citation: Dhamotharan, Karthikeyan; Wacker, Anna; Becker, Matthias; Gunther, Sebastian; Schwalbe, Harald; Korn, Sophie; Schlundt, Andreas. "Conserved residues in the SARS-CoV-2 nucleocapsid N-terminal domain coordinate structural stability and steer RNA recognition" .

Assembly members:

Assembly members:
entity_1, polymer, 138 residues, Formula weight is not available

Natural source:

Natural source: Common Name: SARS-CoV-2 Taxonomy ID: 2697049 Superkingdom: Viruses Kingdom: not available Genus/species: Betacoronavirus HCoV-SARS

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-Sumo

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GGLPNNTASWFTALTQHGKE DLKFSRGQGVPINTNSSPDD QIGYYRRATRRIRGGDGKMK DLSPRWYFYYLGTGPEAGLP YGANKDGIIWVATEGALNTP KDHIGTRNPANNAAIVLQLP QGTTLPKGFYAEGSRGGS

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	380
15N chemical shifts	126
1H chemical shifts	126

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	NTD P67S	1

Entities:

Entity 1, NTD P67S 138 residues - Formula weight is not available

The first G (43) is a cloning artefact. The natural sequence starts from the second G (44).

1

GLY

LEU

PRO

ASN

THR

ALA

SER

TRP

2

PHE

THR

ALA

LEU

THR

GLN

HIS

GLY

LYS

GLU

3

ASP

LEU

LYS

PHE

SER

ARG

GLY

GLN

GLY

VAL

4

PRO

ILE

ASN

THR

ASN

SER

PRO

ASP

5

GLN

ILE

GLY

TYR

ARG

ALA

THR

ARG

6

ARG

ILE

ARG

GLY

ASP

GLY

LYS

MET

LYS

7

ASP

LEU

SER

PRO

ARG

TRP

TYR

PHE

TYR

8

LEU

GLY

THR

GLY

PRO

GLU

ALA

GLY

LEU

PRO

9

TYR

GLY

ALA

ASN

LYS

ASP

GLY

ILE

TRP

10

VAL

ALA

THR

GLU

GLY

ALA

LEU

ASN

THR

PRO

11

LYS

ASP

HIS

ILE

GLY

THR

ARG

ASN

PRO

ALA

12

ASN

ALA

ILE

VAL

LEU

GLN

LEU

PRO

13

GLN

GLY

THR

LEU

PRO

LYS

GLY

PHE

TYR

14

ALA

GLU

GLY

SER

ARG

GLY

SER

Samples:

sample_1: NTD P67S, [U-99% 13C; U-99% 15N], 750 ± 15 uM; potassium phosphate 25 mM; potassium chloride 150 mM; sodium azide 0.02%

sample_conditions_1: ionic strength: 0.15 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN v4 - collection, processing

CcpNMR v2.5, 3.1 - chemical shift assignment, data analysis

NMR spectrometers:

Bruker AVANCE II 600 MHz