BMRB Entry 52544

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52544

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Title:
Backbone resonance assignemnts of HUWE1 HECT domain N-lobe truncation D4141-4216
Deposition date:
2024-07-10
Original release date:
2024-07-16
Authors:
Zhang, Haoran; Li, Yan
Citation:

Citation: Zhang, Haoran; Li, Yan. "Backbone resonance assignemnts of HUWE1 HECT domain N-lobe truncation D4141-4216"  .

Assembly members:

Assembly members:
entity_1, polymer, 195 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GPGSDFDVKRKYFRQELERL DEGLRKEDMAVHVRRDHVFE DSYRELHRKSPEEMKNRLYI VFEGEEGQDAGGLLREWYMI ISREMFNPMYALFRTSPGDR VTYTINPSSHCNPNHLSYFK FVGRIVAKAVYDNRLLECYF TRSFYKHILGKSGGGGSGAI RKQLAAFLEGFYEIIPKRLI SIFTEQELELLISGL

Data typeCount
13C chemical shifts538
15N chemical shifts179
1H chemical shifts179

Additional metadata:

  • Assembly
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Assembly:

Entity Assembly IDEntity NameEntity ID
1HUWE1 HECT domain N-lobe truncation D4141-42161

Entities:

Entity 1, HUWE1 HECT domain N-lobe truncation D4141-4216 195 residues - Formula weight is not available

Original residues 4141-4216 in the full-length HUWE1 HECT domain N-lobe (sequence 'VRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMT') was replaced with a 'GGGGS' linker in this truncated version of HUWE1 HECT domain N-lobe.

1   GLYPROGLYSERASPPHEASPVALLYSARG
2   LYSTYRPHEARGGLNGLULEUGLUARGLEU
3   ASPGLUGLYLEUARGLYSGLUASPMETALA
4   VALHISVALARGARGASPHISVALPHEGLU
5   ASPSERTYRARGGLULEUHISARGLYSSER
6   PROGLUGLUMETLYSASNARGLEUTYRILE
7   VALPHEGLUGLYGLUGLUGLYGLNASPALA
8   GLYGLYLEULEUARGGLUTRPTYRMETILE
9   ILESERARGGLUMETPHEASNPROMETTYR
10   ALALEUPHEARGTHRSERPROGLYASPARG
11   VALTHRTYRTHRILEASNPROSERSERHIS
12   CYSASNPROASNHISLEUSERTYRPHELYS
13   PHEVALGLYARGILEVALALALYSALAVAL
14   TYRASPASNARGLEULEUGLUCYSTYRPHE
15   THRARGSERPHETYRLYSHISILELEUGLY
16   LYSSERGLYGLYGLYGLYSERGLYALAILE
17   ARGLYSGLNLEUALAALAPHELEUGLUGLY
18   PHETYRGLUILEILEPROLYSARGLEUILE
19   SERILEPHETHRGLUGLNGLULEUGLULEU
20   LEUILESERGLYLEU

Related Database Links:

Uniprot Q7Z6Z7

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks