BMRB Entry 52693

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52693

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Title:
NMR signal assignment of the Kinase-Fat linker region (KFL) of the non receptor tyrosine kinase Pyk2
Deposition date:
2024-11-13
Original release date:
2025-05-20
Authors:
Boll, Emmanuelle; Martin, Lucy; Cantrelle, Francois-Xavier; Landrieu, Isabelle
Citation:

Citation: Martin, Lucy; Freitas, Daniela; Boll, Emmanuelle; Brans, Alain; Mortelecque, Justine; Cantrelle, Francois-Xavier; Dourlen, Pierre; Gomes, Claudio; Danis, Clement; Landrieu, Isabelle. "Conformation-Driven Phase Separation in the Linker Domain of Focal Adhesion Kinases"  Biochemistry 64, 1797-1806 (2025).
PubMed: 40136251

Assembly members:

Assembly members:
entity_1, polymer, 197 residues, Formula weight is not available
entity_2, polymer, 43 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MGSSHHHHHHSSGLVPRGSH MQERNARYRTPKILEPTAFQ EPPPKPSRPKYRPPPQTNLL APKLQFQVPEGLCASSPTLT SPMEYPSPVNSLHTPPLHRH NVFKRHSMREEDFIQPSSRE EAQQLWEAEKVKMRQILDKQ QKQMVEDYQWLRQEEKSLDP MVYMNDKSPLTPEKEVGYLE FTGPPQKPPRLGAQSIQ
entity_2: SSREEAQQLWEAEKVKMRQI LDKQQKQMVEDYQWLRQEEK SLD

Data sets:
Data typeCount
13C chemical shifts477
15N chemical shifts158
1H chemical shifts293

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Pyk2 FL1
2Pyk2 Helix2

Entities:

Entity 1, Pyk2 FL 197 residues - Formula weight is not available

1   METGLYSERSERHISHISHISHISHISHIS
2   SERSERGLYLEUVALPROARGGLYSERHIS
3   METGLNGLUARGASNALAARGTYRARGTHR
4   PROLYSILELEUGLUPROTHRALAPHEGLN
5   GLUPROPROPROLYSPROSERARGPROLYS
6   TYRARGPROPROPROGLNTHRASNLEULEU
7   ALAPROLYSLEUGLNPHEGLNVALPROGLU
8   GLYLEUCYSALASERSERPROTHRLEUTHR
9   SERPROMETGLUTYRPROSERPROVALASN
10   SERLEUHISTHRPROPROLEUHISARGHIS
11   ASNVALPHELYSARGHISSERMETARGGLU
12   GLUASPPHEILEGLNPROSERSERARGGLU
13   GLUALAGLNGLNLEUTRPGLUALAGLULYS
14   VALLYSMETARGGLNILELEUASPLYSGLN
15   GLNLYSGLNMETVALGLUASPTYRGLNTRP
16   LEUARGGLNGLUGLULYSSERLEUASPPRO
17   METVALTYRMETASNASPLYSSERPROLEU
18   THRPROGLULYSGLUVALGLYTYRLEUGLU
19   PHETHRGLYPROPROGLNLYSPROPROARG
20   LEUGLYALAGLNSERILEGLN

Entity 2, Pyk2 Helix 43 residues - Formula weight is not available

1   SERSERARGGLUGLUALAGLNGLNLEUTRP
2   GLUALAGLULYSVALLYSMETARGGLNILE
3   LEUASPLYSGLNGLNLYSGLNMETVALGLU
4   ASPTYRGLNTRPLEUARGGLNGLUGLULYS
5   SERLEUASP

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks