BMRB Entry 52794

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52794

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Title:
Drosophila RNA polymerase II Rpb1 CTD 1657-1739
Deposition date:
2025-01-08
Original release date:
2025-07-03
Authors:
Chen, Wei; Showalter, Scott
Citation:

Citation: Chen, Wei; Laremore, Tatiana; Yennawar, Neela; Showalter, Scott. "Phosphorylation patterns modulate the transient secondary structure of RNA polymerase II CTD without altering its global conformation"  .

Assembly members:

Assembly members:
entity_1, polymer, 87 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: fruit fly   Taxonomy ID: 7227   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Drosophila melanogaster

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET His6 MBP TEV LIC cloning vector (1M)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: SNAGFAGSGSNIYSPGNAYS PSSSNYSPNSPSYSPTSPSY SPSSPSYSPTSPCYSPTSPS YSPTSPNYTPVTPSYSPTSP NYSASPQ

Data sets:
Data typeCount
13C chemical shifts289
15N chemical shifts86

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1CTD21

Entities:

Entity 1, CTD2 87 residues - Formula weight is not available

Residues 1-4 (amino acids SNAG) are cloning artifacts remaining after MBP cleavage by TEV.

1   SERASNALAGLYPHEALAGLYSERGLYSER
2   ASNILETYRSERPROGLYASNALATYRSER
3   PROSERSERSERASNTYRSERPROASNSER
4   PROSERTYRSERPROTHRSERPROSERTYR
5   SERPROSERSERPROSERTYRSERPROTHR
6   SERPROCYSTYRSERPROTHRSERPROSER
7   TYRSERPROTHRSERPROASNTYRTHRPRO
8   VALTHRPROSERTYRSERPROTHRSERPRO
9   ASNTYRSERALASERPROGLN