BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5280

Title: Assignments of the 1H, 13C, and 15N, resonances of the winged helix domain of the proto-oncoprotein c-Qin (FoxG1B)

Deposition date: 2002-02-06 Original release date: 2002-08-23

Authors: Jayawardene, Dhammika; Liu, Junmin; Zhang, Weixing; Kriwacki, Richard

Citation: Jayawardene, Dhammika; Liu, Junmin; Zhang, Weixing; Kriwacki, Richard. "Letter to the Editor: Assignments of the 1H, 13C, and 15N, resonances of the winged helix domain of the proto-oncoprotein c-Qin (FoxG1B) "  J. Biomol. NMR 23, 243-244 (2002).

Assembly members:
cellular Qin winged helix domain, polymer, 127 residues, 14720.8 Da.

Natural source:   Common Name: chicken   Taxonomy ID: 9031   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Gallus gallus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET_28a

Entity Sequences (FASTA):
cellular Qin winged helix domain: GSHMGKEGEKKNGKYEKPPF SYNALIMMAIRQSPEKRLTL NGIYEFIMKNFPYYRENKQG WQNSIRHNLSLNKSFVKVPR HYDDPGKGNYWMLDPSSDDV FIGGTTGKLRRRSTTSRAKL AFKRGAR

Data sets:
Data typeCount
1H chemical shifts863
13C chemical shifts548
15N chemical shifts133

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1c-Qin-WHD1

Entities:

Entity 1, c-Qin-WHD 127 residues - 14720.8 Da.

1   GLYSERHISMETGLYLYSGLUGLYGLULYS
2   LYSASNGLYLYSTYRGLULYSPROPROPHE
3   SERTYRASNALALEUILEMETMETALAILE
4   ARGGLNSERPROGLULYSARGLEUTHRLEU
5   ASNGLYILETYRGLUPHEILEMETLYSASN
6   PHEPROTYRTYRARGGLUASNLYSGLNGLY
7   TRPGLNASNSERILEARGHISASNLEUSER
8   LEUASNLYSSERPHEVALLYSVALPROARG
9   HISTYRASPASPPROGLYLYSGLYASNTYR
10   TRPMETLEUASPPROSERSERASPASPVAL
11   PHEILEGLYGLYTHRTHRGLYLYSLEUARG
12   ARGARGSERTHRTHRSERARGALALYSLEU
13   ALAPHELYSARGGLYALAARG

Samples:

15N_13C_labelled: cellular Qin winged helix domain, [U-98% 13C; U-98% 15N], 2.0 mM

15N_labelled: cellular Qin winged helix domain, [U-98% 15N], 2 mM

Cond_1: pH: 5.5; temperature: 313 K; ionic strength: 0.100 M

Experiments:

NameSampleSample stateSample conditions
CBCA(CO)NHnot availablenot availablenot available
HNCACBnot availablenot availablenot available
HN(CO)CAnot availablenot availablenot available
HNCAnot availablenot availablenot available
HNCOnot availablenot availablenot available
HN(CA)COnot availablenot availablenot available
(H)C(CO)NH-TOCSYnot availablenot availablenot available
H(CCO)NH-TOCSYnot availablenot availablenot available
HBHA(CBCACO)NHnot availablenot availablenot available
HCCH-COSYnot availablenot availablenot available
LRCCnot availablenot availablenot available
LRCHnot availablenot availablenot available
15N-edited NOESYnot availablenot availablenot available
13C-edited NOESYnot availablenot availablenot available
aromatic- HB(CBCG)CDHDnot availablenot availablenot available
(HB)CB(CG)CDHDnot availablenot availablenot available
HB(CBCECD)CEHEnot availablenot availablenot available
(HB)CB(CGCD)CEHE edited 3D NOESYnot availablenot availablenot available
(HB)CB(CGCD)CEHE edited 4D NOESYnot availablenot availablenot available

Software:

Felix v98 - NMR data processing, analysis

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

DBJ BAE21092
EMBL CAA52239 CAF93027 CAI91293
GB AAA40812 AAA66954 AAB08466 AAB42158 AAC18393
PIR A47446 A54743
PRF 2115219B
REF NP_001079165 NP_001116933 NP_001153584 NP_005240 NP_032267
SP P55316 P56260 Q00939 Q1A1A1 Q1A1A2
TPG DAA17496
AlphaFold P55316 Q1A1A2 Q1A1A1 Q00939 P56260

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts