BMRB Entry 52830

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52830

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Title:
Functionally Distinct SNARE Motifs of SNAP25 Cooperate in SNARE Assembly and Membrane Fusion: SNAP25-4S
Deposition date:
2025-01-12
Original release date:
2025-01-28
Authors:
Kraichely, Katelyn; Sandall, Connor; Liang, Binyong; Kiessling, Volker; Tamm, Lukas
Citation:

Citation: Kraichely, Katelyn; Sandall, Connor; Liang, Binyong; Kiessling, Volker; Tamm, Lukas. "Functionally distinct SNARE motifs of SNAP25 cooperate in SNARE assembly and membrane fusion"  Biophys. J. 124, 637-650 (2025).
PubMed: 39982442

Assembly members:

Assembly members:
entity_1, polymer, 206 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MAEDADMRNELEEMQRRADQ LADESLESTRRMLQLVEESK DAGIRTLVMLDEQGEQLDRV EEGMNHINQDMKEAEKNLKD LGKSSGLFISPSNKLKSSDA YKKAWGNNQDGVVASQPARV VDEREQMAISGGFIRRVTND ARENEMDENLEQVSGIIGNL RHMALDMGNEIDTQNRQIDR IMEKADSNKTRIDEANQRAT KMLGSG

Data sets:
Data typeCount
13C chemical shifts596
15N chemical shifts201
1H chemical shifts201

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1SNAP25-4S1

Entities:

Entity 1, SNAP25-4S 206 residues - Formula weight is not available

1   METALAGLUASPALAASPMETARGASNGLU
2   LEUGLUGLUMETGLNARGARGALAASPGLN
3   LEUALAASPGLUSERLEUGLUSERTHRARG
4   ARGMETLEUGLNLEUVALGLUGLUSERLYS
5   ASPALAGLYILEARGTHRLEUVALMETLEU
6   ASPGLUGLNGLYGLUGLNLEUASPARGVAL
7   GLUGLUGLYMETASNHISILEASNGLNASP
8   METLYSGLUALAGLULYSASNLEULYSASP
9   LEUGLYLYSSERSERGLYLEUPHEILESER
10   PROSERASNLYSLEULYSSERSERASPALA
11   TYRLYSLYSALATRPGLYASNASNGLNASP
12   GLYVALVALALASERGLNPROALAARGVAL
13   VALASPGLUARGGLUGLNMETALAILESER
14   GLYGLYPHEILEARGARGVALTHRASNASP
15   ALAARGGLUASNGLUMETASPGLUASNLEU
16   GLUGLNVALSERGLYILEILEGLYASNLEU
17   ARGHISMETALALEUASPMETGLYASNGLU
18   ILEASPTHRGLNASNARGGLNILEASPARG
19   ILEMETGLULYSALAASPSERASNLYSTHR
20   ARGILEASPGLUALAASNGLNARGALATHR
21   LYSMETLEUGLYSERGLY

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