BMRB Entry 5296
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR5296
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Title: 1H Resonance Assignments at 310K and pH 4.90 for E.coli recombinant Brazzein variant brazzein-ins(R18a-I18b), an Non-Sweet variant of Brazzein
Deposition date: 2002-01-18 Original release date: 2005-09-22
Authors: Zhao, Qin; Song, Jikui; Markley, John
Citation: Zhao, Qin; Song, Jikui; Jin, Zheyuan; Danilova, Vicktoria; Hellekant, Goran; Markley, John. "Probing the sweet determinants of brazzein: Wild-type brazzein and a tasteless variant, brazzein-ins(R(18a)-I(18b)), exhibit different pH-dependent NMR chemical shifts" Biochem. Biophys. Res. Commun. 335, 256-263 (2005).
Assembly members:
brazzein-ins(R18a-I18b), polymer, 55 residues, Formula weight is not available
Natural source: Common Name: Joublie fruit Taxonomy ID: 43545 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Pentadiplandra brazzeana
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
brazzein-ins(R18a-I18b): DKCKKVYENYPVSKCQLRIA
NQCNYDCKLDKHARSGECFY
DEKRNLQCICDYCEY
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 255 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | brazzein-ins-(R18a-I18b) | 1 |
Entities:
Entity 1, brazzein-ins-(R18a-I18b) 55 residues - Formula weight is not available
| 1 | ASP | LYS | CYS | LYS | LYS | VAL | TYR | GLU | ASN | TYR | ||||
| 2 | PRO | VAL | SER | LYS | CYS | GLN | LEU | ARG | ILE | ALA | ||||
| 3 | ASN | GLN | CYS | ASN | TYR | ASP | CYS | LYS | LEU | ASP | ||||
| 4 | LYS | HIS | ALA | ARG | SER | GLY | GLU | CYS | PHE | TYR | ||||
| 5 | ASP | GLU | LYS | ARG | ASN | LEU | GLN | CYS | ILE | CYS | ||||
| 6 | ASP | TYR | CYS | GLU | TYR |
Samples:
sample_1: brazzein-ins(R18a-I18b) 1 mM; KCl 100 mM; DSS 0.1 mM
conditions_set_1: ionic strength: 100 mM; pH: 4.90; temperature: 310 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| TOCSY | sample_1 | not available | conditions_set_1 |
Software:
No software information available
NMR spectrometers:
- Bruker DMX 500 MHz