BMRB Entry 5306
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5306
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
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All files associated with the entry
Citation: Freedman, Steven; Sun, Zhen-Yu; Poy, Florence; Kung, Andrew; Livingston, David; Wagner, Gerhard; Eck, Michael. "Structural Basis for Recruitment of CBP/p300 by Hypoxia-inducible Factor-1 alpha
" Proc. Natl. Acad. Sci. U. S. A. 99, 5367-5372 (2002).
PubMed: 11959990
Assembly members:
C-terminal activation domain of hypoxia inducible factor-1alpha, polymer, 42 residues, Formula weight is not available
p300 cysteine/histidine-rich 1 domain, polymer, 101 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
C-terminal activation domain of hypoxia inducible factor-1alpha: GSMDESGLPQLTSYDCEVNA
PIQGSRNLLQGEELLRALDQ
VN
p300 cysteine/histidine-rich 1 domain: MGSGAHTADPEKRKLIQQQL
VLLLHAHKCQRREQANGEVR
QCNLPHCRTMKNVLNHMTHC
QSGKSCQVAHCASSRQIISH
WKNCTRHDCPVCLPLKNAGD
K
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 805 |
| 13C chemical shifts | 557 |
| 15N chemical shifts | 146 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | HIF-1alpha C-terminal activation domain | 1 |
| 2 | p300 cysteine/histidine-rich 1 domain | 2 |
| 3 | ZINC (II) ION, I | 3 |
| 4 | ZINC (II) ION, II | 3 |
| 5 | ZINC (II) ION, III | 3 |
Entities:
Entity 1, HIF-1alpha C-terminal activation domain 42 residues - Formula weight is not available
| 1 | GLY | SER | MET | ASP | GLU | SER | GLY | LEU | PRO | GLN | ||||
| 2 | LEU | THR | SER | TYR | ASP | CYS | GLU | VAL | ASN | ALA | ||||
| 3 | PRO | ILE | GLN | GLY | SER | ARG | ASN | LEU | LEU | GLN | ||||
| 4 | GLY | GLU | GLU | LEU | LEU | ARG | ALA | LEU | ASP | GLN | ||||
| 5 | VAL | ASN |
Entity 2, p300 cysteine/histidine-rich 1 domain 101 residues - Formula weight is not available
| 1 | MET | GLY | SER | GLY | ALA | HIS | THR | ALA | ASP | PRO | ||||
| 2 | GLU | LYS | ARG | LYS | LEU | ILE | GLN | GLN | GLN | LEU | ||||
| 3 | VAL | LEU | LEU | LEU | HIS | ALA | HIS | LYS | CYS | GLN | ||||
| 4 | ARG | ARG | GLU | GLN | ALA | ASN | GLY | GLU | VAL | ARG | ||||
| 5 | GLN | CYS | ASN | LEU | PRO | HIS | CYS | ARG | THR | MET | ||||
| 6 | LYS | ASN | VAL | LEU | ASN | HIS | MET | THR | HIS | CYS | ||||
| 7 | GLN | SER | GLY | LYS | SER | CYS | GLN | VAL | ALA | HIS | ||||
| 8 | CYS | ALA | SER | SER | ARG | GLN | ILE | ILE | SER | HIS | ||||
| 9 | TRP | LYS | ASN | CYS | THR | ARG | HIS | ASP | CYS | PRO | ||||
| 10 | VAL | CYS | LEU | PRO | LEU | LYS | ASN | ALA | GLY | ASP | ||||
| 11 | LYS |
Entity 3, ZINC (II) ION, I - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: C-terminal activation domain of hypoxia inducible factor-1alpha 1.0 mM; p300 cysteine/histidine-rich 1 domain 1.0 mM; ZnSO4 0.1 mM; DTT 1 mM; NaCl 100 mM
sample_2: C-terminal activation domain of hypoxia inducible factor-1alpha, [U-15N], 1.0 mM; p300 cysteine/histidine-rich 1 domain, [U-15N], 1.0 mM; ZnSO4 0.1 mM; DTT 1 mM; NaCl 100 mM
sample_3: C-terminal activation domain of hypoxia inducible factor-1alpha, [U-13C; U-15N], 1.0 mM; p300 cysteine/histidine-rich 1 domain, [U-13C; U-15N], 1.0 mM; ZnSO4 0.1 mM; DTT 1 mM; NaCl 100 mM
Condition_1: pH: 6.0; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| not available | not available | not available | Condition_1 |
Software:
No software information available
NMR spectrometers:
- Bruker Avance 500 MHz
- Varian UnityInova 750 MHz
- Varian Unity 500 MHz
Related Database Links:
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks