BMRB Entry 5311
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5311
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Title: Letter to the Editor: 1H, 15N and 13C assignments of the Pseudomonas effector protein, AvrPto
Deposition date: 2002-03-06 Original release date: 2002-08-23
Authors: Wulf, Jennifer; Pascuzzi, Pete; Martin, Gregory; Nicholson, Linda
Citation: Wulf, Jennifer; Pascuzzi, Pete; Martin, Gregory; Nicholson, Linda. "Letter to the Editor: 1H, 15N and 13C assignments of the Pseudomonas effector protein, AvrPto " J. Biomol. NMR 23, 247-248 (2002).
Assembly members:
AvrPto, polymer, 105 residues, Formula weight is not available
Natural source: Common Name: Pseudomonas syringae Taxonomy ID: 317 Superkingdom: Eubacteria Kingdom: not available Genus/species: Pseudomonas syringae
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
AvrPto: DNVTSSQLLSVRHQLAESAG
LPRDQHEFVSSQAPQSLRNR
YNNLYSHTQRTLDMADMQHR
YMTGASGINPGMLPHENVDD
MRSAITDWSDMREALQHAMG
IHADI
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 629 |
| 13C chemical shifts | 389 |
| 15N chemical shifts | 101 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | AvrPto subunit 1 | 1 |
| 2 | AvrPto subunit 2 | 1 |
Entities:
Entity 1, AvrPto subunit 1 105 residues - Formula weight is not available
| 1 | ASP | ASN | VAL | THR | SER | SER | GLN | LEU | LEU | SER | ||||
| 2 | VAL | ARG | HIS | GLN | LEU | ALA | GLU | SER | ALA | GLY | ||||
| 3 | LEU | PRO | ARG | ASP | GLN | HIS | GLU | PHE | VAL | SER | ||||
| 4 | SER | GLN | ALA | PRO | GLN | SER | LEU | ARG | ASN | ARG | ||||
| 5 | TYR | ASN | ASN | LEU | TYR | SER | HIS | THR | GLN | ARG | ||||
| 6 | THR | LEU | ASP | MET | ALA | ASP | MET | GLN | HIS | ARG | ||||
| 7 | TYR | MET | THR | GLY | ALA | SER | GLY | ILE | ASN | PRO | ||||
| 8 | GLY | MET | LEU | PRO | HIS | GLU | ASN | VAL | ASP | ASP | ||||
| 9 | MET | ARG | SER | ALA | ILE | THR | ASP | TRP | SER | ASP | ||||
| 10 | MET | ARG | GLU | ALA | LEU | GLN | HIS | ALA | MET | GLY | ||||
| 11 | ILE | HIS | ALA | ASP | ILE |
Samples:
sample_1: AvrPto, [U-15N], 1.0 mM
sample_2: AvrPto, [U-13C; U-15N], 1.0 mM
condition_all: pH: 6.0 na; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N HSQC | not available | not available | not available |
| 2D homonuclear NOESY | not available | not available | not available |
| 2D homonuclear TOCSY | not available | not available | not available |
| 1H-15N NOESY-HSQC | not available | not available | not available |
| 1H-15N TOCSY-HSQC | not available | not available | not available |
| HNCA | not available | not available | not available |
| HNCO | not available | not available | not available |
| HC(CO)NH | not available | not available | not available |
| C(CO)NH | not available | not available | not available |
| CBCANH | not available | not available | not available |
| CBCA(CO)NH | not available | not available | not available |
| 1H-13C HSQC | not available | not available | not available |
| 1H-13C NOESY | not available | not available | not available |
| 1H-13C aromatic HSQC | not available | not available | not available |
| 1H-13C HCCH-TOCSY | not available | not available | not available |
Software:
No software information available
NMR spectrometers:
- Varian Inova 600 MHz
Related Database Links:
| PDB | |
| GB | AAA25728 AAO57459 ABS87317 ABS87322 AHN52240 |
| PRF | 1916396A |
| REF | NP_793764 WP_011104823 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts