BMRB Entry 5325
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR5325
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Title: 1H Chemical Shift Assignments for nsLTP2 from Rice PubMed: 12011089
Deposition date: 2002-03-18 Original release date: 2003-06-09
Authors: Ping-Chiang, Lyu
Citation: Samuel, Dharmaraj; Liu, Yaw-Jen; Cheng, Chao-Sheng; Lyu, Ping-Chiang. "Solution Structure of Plant Nonspecific Lipid Transfer Protein-2 from Rice (Oryza sativa)" J. Biol. Chem. 277, 35267-35273 (2002).
Assembly members:
Plant Lipid Transfer Protein-2, polymer, 69 residues, 7001 Da.
Natural source: Common Name: Rice Taxonomy ID: 4530 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Oryza sativa
Experimental source: Production method: purified from the natural source
Entity Sequences (FASTA):
Plant Lipid Transfer Protein-2: AGCNAGQLTVCTGAIAGGAR
PTAACCSSLRAQQGCFCQFA
KDPRYGRYVNSPNARKAVSS
CGIALPTCH
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 385 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | rice nsLTP2 | 1 |
Entities:
Entity 1, rice nsLTP2 69 residues - 7001 Da.
| 1 | ALA | GLY | CYS | ASN | ALA | GLY | GLN | LEU | THR | VAL | ||||
| 2 | CYS | THR | GLY | ALA | ILE | ALA | GLY | GLY | ALA | ARG | ||||
| 3 | PRO | THR | ALA | ALA | CYS | CYS | SER | SER | LEU | ARG | ||||
| 4 | ALA | GLN | GLN | GLY | CYS | PHE | CYS | GLN | PHE | ALA | ||||
| 5 | LYS | ASP | PRO | ARG | TYR | GLY | ARG | TYR | VAL | ASN | ||||
| 6 | SER | PRO | ASN | ALA | ARG | LYS | ALA | VAL | SER | SER | ||||
| 7 | CYS | GLY | ILE | ALA | LEU | PRO | THR | CYS | HIS |
Samples:
sample_1: Plant Lipid Transfer Protein-2 3.0 mM
Cond_I: pH: 6.4; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| DQF-COSY | not available | not available | not available |
| TOCSY | not available | not available | not available |
| NOESY | not available | not available | not available |
Software:
Xwin-nmr v2.1 -
NMR spectrometers:
- Bruker Avance 600 MHz