BMRB Entry 5344

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR5344

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

1H, 15N Assignment of Neocarzinostatin Apo-Protein Bound to a Synthetic Chromophore

Deposition date:

2002-04-12

Original release date:

2006-04-05

Authors:

Urbaniak, Michael; Muskett, Frederick; Finucane, Michael; Caddick, Stephen; Woolfson, Derek

Citation:

Citation: Urbaniak, Michael; Muskett, Frederick; Finucane, M.; Caddick, Stephen; Woolfson, Derek. "Solution Structure of a Novel Chromoprotein dervived from apo-Neocarzinostatin and a Synthetic Chromophore " Biochemistry 41, 11731-11739 (2002).
PubMed: 12269815

Assembly members:

Assembly members:
Neocarzinostatin apo-protein, polymer, 122 residues, 12213 Da.
SM, non-polymer, 150.360 Da.

Natural source:

Natural source: Common Name: Streptomyces carzinostaticus Taxonomy ID: 1897 Superkingdom: Eubacteria Kingdom: not available Genus/species: Streptomyces carzinostaticus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pCANTABB

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Neocarzinostatin apo-protein: HHHHHHLQGAAPTATVTPSS GLSDGTVVKVAGAGLQAGTA YDVGQCAWVDTGVLACNPAD FSSVTADANGSASTSLTVRR SFEGFLFDGTRWGTVDCTTA ACQVGLSDAAGNGPEGVAIS FN

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
15N chemical shifts	113
1H chemical shifts	552

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Neocarzinostatin apo-protein	1
2	Napthoate ester	2

Entities:

Entity 1, Neocarzinostatin apo-protein 122 residues - 12213 Da.

1

HIS

LEU

GLN

GLY

ALA

2

ALA

PRO

THR

ALA

THR

VAL

THR

PRO

SER

3

GLY

LEU

SER

ASP

GLY

THR

VAL

LYS

VAL

4

ALA

GLY

ALA

GLY

LEU

GLN

ALA

GLY

THR

ALA

5

TYR

ASP

VAL

GLY

GLN

CYS

ALA

TRP

VAL

ASP

6

THR

GLY

VAL

LEU

ALA

CYS

ASN

PRO

ALA

ASP

7

PHE

SER

VAL

THR

ALA

ASP

ALA

ASN

GLY

8

SER

ALA

SER

THR

SER

LEU

THR

VAL

ARG

9

SER

PHE

GLU

GLY

PHE

LEU

PHE

ASP

GLY

THR

10

ARG

TRP

GLY

THR

VAL

ASP

CYS

THR

ALA

11

ALA

CYS

GLN

VAL

GLY

LEU

SER

ASP

ALA

12

GLY

ASN

GLY

PRO

GLU

GLY

VAL

ALA

ILE

SER

13

PHE

ASN

Entity 2, Napthoate ester - Sm - 150.360 Da.

1

SM

Samples:

sample_1: Neocarzinostatin apo-protein, [U-15N], 1.0 mM; 2-hydroxy-7-methoxy-5-methyl-naphthalene-1-carboxylic acid meso-2 5-dihydroxy-cyclopent-3-enyl ester 1.0 mM; D2O 10 % v/v; phosphate buffer 25 mM; TSP 0.1 mM; sodium azide 0.005 % w/w

condition_1: pH: 5.0; temperature: 308 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 1H-1H-15N TOCSY	sample_1	not available	condition_1
3D 1H-1H-15N NOESY	sample_1	not available	condition_1
3D HNHA	sample_1	not available	condition_1
3D HNHB	sample_1	not available	condition_1
2D 1H-15N HSQC	sample_1	not available	condition_1
2D 1H-1H NOESY	sample_1	not available	condition_1
HNHB	sample_1	not available	condition_1
NOESY	sample_1	not available	condition_1

Software:

NMRpipe - processing

XEASY - on-screen assignments

DYANA - Structure calculation

NMR spectrometers:

Varian INOVA 600 MHz
Varian UnityPlus 500 MHz

BMRB	1766 5343 5969 6888 695
PDB	1J5H 1J5I 1NCO 1NOA 1O5P 2G0K 2G0L
DBJ	BAA01764 BAB83894
GB	AAB28103 AAM77994
SP	P0A3R9 P0A3S0
AlphaFold	P0A3R9 P0A3S0