BMRB Entry 5464
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5464
MolProbity Validation Chart
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NMR-STAR v3 text file.
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Citation: Kwan, A.; Gell, D.; Liew, C.; Mackay, J.. "Solution Structure of a CCHC Zinc Finger from MOZ" The BMRB entry is the only known published source for the data..
Assembly members:
Zinc finger protein, polymer, 31 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: PGEX-2T
Entity Sequences (FASTA):
Zinc finger protein: RLPKLYLCEFCLKYMKSRTI
LQQHMKKCGWF
- assigned_chemical_shifts
- coupling_constants
| Data type | Count |
| 1H chemical shifts | 216 |
| coupling constants | 5 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Monocytic leukemia zinc finger protein | 1 |
| 2 | Zinc ion | 2 |
Entities:
Entity 1, Monocytic leukemia zinc finger protein 31 residues - Formula weight is not available
| 1 | ARG | LEU | PRO | LYS | LEU | TYR | LEU | CYS | GLU | PHE | ||||
| 2 | CYS | LEU | LYS | TYR | MET | LYS | SER | ARG | THR | ILE | ||||
| 3 | LEU | GLN | GLN | HIS | MET | LYS | LYS | CYS | GLY | TRP | ||||
| 4 | PHE |
Entity 2, Zinc ion - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: Zinc finger protein 0.5 mM; ZINC (II) ION 0.7 mM; TCEP 1 mM; H2O 95%; D2O 5%
sample_cond_1: pH: 5.6; temperature: 298 K; pressure: 1 atm
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D NOESY | sample_1 | not available | sample_cond_1 |
| 2D TOCSY | sample_1 | not available | sample_cond_1 |
| DQF-COSY | sample_1 | not available | sample_cond_1 |
Software:
XWINNMR v2.5 - processing
XEASY v1.3.13 - data analysis
DYANA v1.5 - refinement
ARIA v1.1.2 - structure solution
NMR spectrometers:
- Bruker DRX 600 MHz