BMRB Entry 5525
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5525
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Title: NMR structure of the first Zinc Binding domain of Nup475/TTP/TIS11 PubMed: 12515557
Deposition date: 2002-09-13 Original release date: 2003-09-05
Authors: Amann, B.; Guerrerio, A.; Pelo, J.; Luo, R.; Worthington, M.; Berg, J.
Citation: Amann, B.; Worthington, M.; Berg, J.. "A Cys3His Zinc-binding Domain from Nup475/Tristetraprolin: A Novel Fold with a Disklike Structure " Biochemistry 42, 217-221 (2003).
Assembly members:
Tristetraproline, polymer, 77 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Tristetraproline: GSHMTTSSRYKTELCRTYSE
SGRCRYGAKCQFAHGLGELR
QANRHPKYKTELCHKFKLQG
RCPYGSRCHFIHNPTED
- assigned_chemical_shifts
- coupling_constants
| Data type | Count |
| 1H chemical shifts | 220 |
| 13C chemical shifts | 72 |
| coupling constants | 15 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Tristetraproline | 1 |
| 2 | ZINC ION | 2 |
Entities:
Entity 1, Tristetraproline 77 residues - Formula weight is not available
| 1 | GLY | SER | HIS | MET | THR | THR | SER | SER | ARG | TYR | ||||
| 2 | LYS | THR | GLU | LEU | CYS | ARG | THR | TYR | SER | GLU | ||||
| 3 | SER | GLY | ARG | CYS | ARG | TYR | GLY | ALA | LYS | CYS | ||||
| 4 | GLN | PHE | ALA | HIS | GLY | LEU | GLY | GLU | LEU | ARG | ||||
| 5 | GLN | ALA | ASN | ARG | HIS | PRO | LYS | TYR | LYS | THR | ||||
| 6 | GLU | LEU | CYS | HIS | LYS | PHE | LYS | LEU | GLN | GLY | ||||
| 7 | ARG | CYS | PRO | TYR | GLY | SER | ARG | CYS | HIS | PHE | ||||
| 8 | ILE | HIS | ASN | PRO | THR | GLU | ASP |
Entity 2, ZINC ION - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: Tristetraproline 1.7 mM; ZnCl2 3.74 mM; Tris, [U-2H], 50 mM; H20 90%; D20 10%
sample_2: Tristetraproline 1.7 mM; ZnCl2 3.74 mM; Tris, [U-2H], 50 mM; D20 100%
sample_cond_1: pH: 5.8; temperature: 293 K; pressure: 1 atm
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D NOESY | not available | not available | sample_cond_1 |
| 2D TOCSY | not available | not available | sample_cond_1 |
| DQF-COSY | not available | not available | sample_cond_1 |
| 3D 13C-separated NOESY | not available | not available | sample_cond_1 |
| 3D 15N-separated NOESY | not available | not available | sample_cond_1 |
| HNCACB | not available | not available | sample_cond_1 |
| 15N-HSQC-TOCSY | not available | not available | sample_cond_1 |
| 13C-HCCH-TOCSY | not available | not available | sample_cond_1 |
| HSQCJ WEX | not available | not available | sample_cond_1 |
Software:
FELIX v98 - processing
CNS v1.0 - structure solution, refinement
VNMR v6.1b - collection
NMR spectrometers:
- Varian UNITYplus 500 MHz