BMRB Entry 5550
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5550
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Title: Structure and Dynamics of Micelle-bound Neuropeptide Y: Comparison with unligated NPY and Implications for Receptor Selection PubMed: 11124908
Deposition date: 2002-10-07 Original release date: 2008-07-17
Authors: Bader, R.; Bettio, A.; Folkers, G.; Beck-Sickinger, A.G.; Zerbe, O.
Citation: Bader, R.; Bettio, A.; Beck-Sickinger, A.G.; Zerbe, O.. "Structure and Dynamics of Micelle-bound Neuropeptide Y: Comparison with Unligated NPY and Implications for Receptor Selection" J. Mol. Biol. 305, 307-329 (2001).
Assembly members:
Neuropeptide Y, polymer, 37 residues, 4240 Da.
Natural source: Common Name: Pig Taxonomy ID: 9823 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Sus scrofa
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
Neuropeptide Y: YPSKPDNPGEDAPAEDLARY
YSALRHYINLITRQRYX
- assigned_chemical_shifts
- order_parameters
- heteronucl_T1_relaxation
- heteronucl_T2_relaxation
- heteronucl_NOEs
Data type | Count |
1H chemical shifts | 260 |
15N chemical shifts | 64 |
T1 relaxation values | 93 |
T2 relaxation values | 112 |
heteronuclear NOE values | 62 |
order parameters | 27 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | Neuropeptide Y | 1 |
Entities:
Entity 1, Neuropeptide Y 37 residues - 4240 Da.
1 | TYR | PRO | SER | LYS | PRO | ASP | ASN | PRO | GLY | GLU | ||||
2 | ASP | ALA | PRO | ALA | GLU | ASP | LEU | ALA | ARG | TYR | ||||
3 | TYR | SER | ALA | LEU | ARG | HIS | TYR | ILE | ASN | LEU | ||||
4 | ILE | THR | ARG | GLN | ARG | TYR | NH2 |
Samples:
sample_1: Neuropeptide Y, [U-95% 15N], 3 mM; DPC 0.30 M; H2O 90%; D2O 10%
sample_2: Neuropeptide Y, [U-95% 15N], 1 mM; H2O 90%; D2O 10%
Condition_1: pH: 6.0; temperature: 310 K; ionic strength: 0.15 M
Condition_2: pH: 3.1; temperature: 305 K; ionic strength: 0.15 M
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
TOCSY | not available | not available | not available |
NOESY | not available | not available | not available |
DQF-COSY | not available | not available | not available |
Software:
X-PLOR v3.0 - structure calculation
NMR spectrometers:
- Varian UnityPlus 500 MHz
- Bruker AVANCE 600 MHz
Related Database Links:
BMRB | 548 |
PDB | |
DBJ | BAB22495 BAG35090 BAG82945 BAJ20461 |
EMBL | CAD10677 |
GB | AAA41722 AAA41723 AAA41724 AAA59944 AAA59946 |
PRF | 1006203A 1407279B 1612231A 1907264A |
REF | NP_000896 NP_001009452 NP_001014845 NP_001027986 NP_001153758 |
SP | P01303 P01304 P07808 P14765 P57774 |
TPG | DAA30542 |
AlphaFold | P07808 P57774 P14765 P01304 P01303 |
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SPARKY: Backbone
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