BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5572

Title: Beta-helix structure and ice-binding properties of a hyperactive antifreeze protein from an insect   PubMed: 10917537

Deposition date: 2002-11-04 Original release date: 2002-12-27

Authors: Graether, Steffen; Kuiper, Michael; Gagne, Stephane; Walker, Virginia; Jia, Zongchao; Sykes, Brian; Davies, Peter

Citation: Graether, Steffen; Kuiper, Michael; Gagne, Stephane; Walker, Virginia; Jia, Zongchao; Sykes, Brian; Davies, Peter. "Beta-helix structure and ice-binding properties of a hyperactive antifreeze protein from an insect"  Nature 406, 325-328 (2000).

Assembly members:
spruce budworm antifreeze protein, polymer, 90 residues, Formula weight is not available

Natural source:   Common Name: Spruce budworm   Taxonomy ID: 7141   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Choristoneura fumiferana

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
spruce budworm antifreeze protein: DGSCTNTNSQLSANSKCEKS TLTNCYVDKSEVYGTTCTGS RFDGVTITTSTSTGSRISGP GCKISTCIITGGVPAPSAAC KISGCTFSAN

Data sets:
Data typeCount
13C chemical shifts226
15N chemical shifts93
1H chemical shifts495

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1sbwAFP1

Entities:

Entity 1, sbwAFP 90 residues - Formula weight is not available

1   ASPGLYSERCYSTHRASNTHRASNSERGLN
2   LEUSERALAASNSERLYSCYSGLULYSSER
3   THRLEUTHRASNCYSTYRVALASPLYSSER
4   GLUVALTYRGLYTHRTHRCYSTHRGLYSER
5   ARGPHEASPGLYVALTHRILETHRTHRSER
6   THRSERTHRGLYSERARGILESERGLYPRO
7   GLYCYSLYSILESERTHRCYSILEILETHR
8   GLYGLYVALPROALAPROSERALAALACYS
9   LYSILESERGLYCYSTHRPHESERALAASN

Samples:

sample_1: spruce budworm antifreeze protein, [U-15N; U-13C], 18 mM

condition_1: pH: 5.5; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions

Software:

No software information available

NMR spectrometers:

  • unknown unknown 0 MHz

Related Database Links:

BMRB 5573
PDB
GB AAG32660 AAG33032 AAG33033

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts