BMRB Entry 5594

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5594

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Title:
Structural insights into the U-box, a domain associated with multi-ubiquitination
Deposition date:
2002-11-21
Original release date:
2003-04-22
Authors:
Ohi, Melanie; Vander Kooi, Craig; Rosenberg, Joshua; Chazin, Walter; Gould, Kathleen
Citation:

Citation: Ohi, Melanie; Vander Kooi, Craig; Rosenberg, Joshua; Chazin, Walter; Gould, Kathleen. "Structural Insights into the U-box, a Domain Associated with Multi-ubiquitination"  Nat. Struct. Biol. 10, 250-255 (2003).
PubMed: 12627222

Assembly members:

Assembly members:
Prp19, polymer, 52 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Prp19: MLCAISGKVPRRPVLSPKSR TIFEKSLLEQYVKDTGNDPI TNEPLSIEEIVE

Data sets:
  • assigned_chemical_shifts
Data typeCount
13C chemical shifts141
15N chemical shifts46
1H chemical shifts313

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Prp19(1-73), ubox1

Entities:

Entity 1, Prp19(1-73), ubox 52 residues - Formula weight is not available

1   METLEUCYSALAILESERGLYLYSVALPRO
2   ARGARGPROVALLEUSERPROLYSSERARG
3   THRILEPHEGLULYSSERLEULEUGLUGLN
4   TYRVALLYSASPTHRGLYASNASPPROILE
5   THRASNGLUPROLEUSERILEGLUGLUILE
6   VALGLU

Samples:

sample_1: Prp19, [U-13C; U-15N], 1.0 mM; sodium phosphate 20 mM; NaCl 20 mM

sample_2: Prp19, [U-15N], 0.75 mM; sodium phosphate 20 mM; NaCl 20 mM

sample_3: Prp19 1.0 mM; sodium phosphate 20 mM; NaCl 20 mM

Cond_1: ionic strength: 0.28 M; pH: 7.0; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
HNCAnot availablenot availableCond_1
HN(CO)CAnot availablenot availableCond_1
HNCACBnot availablenot availableCond_1
CBCA(CO)NHnot availablenot availableCond_1
1H-1H NOEnot availablenot availableCond_1
1H-1H COSYnot availablenot availableCond_1
1H-1H TOCSYnot availablenot availableCond_1
1H-15N HSQCnot availablenot availableCond_1
1H-1H-15N NOEnot availablenot availableCond_1
1H-1H-15N TOCSYnot availablenot availableCond_1
1H-1H-13C NOEnot availablenot availableCond_1
1H-1H-13C TOCSYnot availablenot availableCond_1

Software:

xwinnmr v2.6 - data processing

XEASY v1.3.13 - assignment, peak integration

DYANA v1.5 - calibration, structure calculation

AMBER v6 - restrained molecular dynamics

NMR spectrometers:

  • Bruker Avance 500 MHz
  • Bruker Avance 600 MHz
  • Bruker Avance 800 MHz

Related Database Links:

PDB
DBJ GAA24853
EMBL CAA68103 CAA97487 CAY81203
GB AAA34912 AAM90948 AHY78374 EDN59512 EDV09279
REF NP_013064
SP P32523
TPG DAA09285
AlphaFold P32523

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks