BMRB Entry 5595

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5595

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Title:
Letter to the Editor: 1H, 13C, 15N backbone resonance assignments of apo-NosL, a novel 18.5 kDa copper(I) binding protein from the nitrous oxide reductase gene cluster of Achromobacter cycloclastes
Deposition date:
2002-11-21
Original release date:
2004-03-15
Authors:
Taubner, Lara; McGuirl, Michele; Dooley, David; Copie, Valerie
Citation:

Citation: Taubner, Lara; McGuirl, Michele; Dooley, David; Copie, Valerie. "Letter to the Editor: 1H, 13C, 15N backbone and sidechain resonance assignments of apo-NosL, a novel copper(I) binding protein from the nitrous oxide reductase gene cluster of Achromobacter cycloclastes"  J. Biomol. NMR 29, 211-212 (2004).
PubMed: 15014237

Assembly members:

Assembly members:
NosL, polymer, 175 residues, 18538 Da.

Natural source:

Natural source:   Common Name: Denitrifying bacteria   Taxonomy ID: 223   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Achromobacter cycloclastes

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-20b+

Entity Sequences (FASTA):

Entity Sequences (FASTA):
NosL: MDKEDVAQSIVPQDMTPETL GHYCQMNLLEHPGPKAQIFL EGSPAPLFFSQVRDAIAYAR GPEQIAPILVIYVNDMGAAG ATWDQPGDGNWIAADKAFYV VGSARRGGMGAPEAVPFSSR DEAAAFVLAEGGQVLALADI TDAMVLTPVETGSEPRADDE DYLGRLRALPHPAGG

Data sets:
Data typeCount
1H chemical shifts846
13C chemical shifts596
15N chemical shifts148

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1NosL monomer1

Entities:

Entity 1, NosL monomer 175 residues - 18538 Da.

1   METASPLYSGLUASPVALALAGLNSERILE
2   VALPROGLNASPMETTHRPROGLUTHRLEU
3   GLYHISTYRCYSGLNMETASNLEULEUGLU
4   HISPROGLYPROLYSALAGLNILEPHELEU
5   GLUGLYSERPROALAPROLEUPHEPHESER
6   GLNVALARGASPALAILEALATYRALAARG
7   GLYPROGLUGLNILEALAPROILELEUVAL
8   ILETYRVALASNASPMETGLYALAALAGLY
9   ALATHRTRPASPGLNPROGLYASPGLYASN
10   TRPILEALAALAASPLYSALAPHETYRVAL
11   VALGLYSERALAARGARGGLYGLYMETGLY
12   ALAPROGLUALAVALPROPHESERSERARG
13   ASPGLUALAALAALAPHEVALLEUALAGLU
14   GLYGLYGLNVALLEUALALEUALAASPILE
15   THRASPALAMETVALLEUTHRPROVALGLU
16   THRGLYSERGLUPROARGALAASPASPGLU
17   ASPTYRLEUGLYARGLEUARGALALEUPRO
18   HISPROALAGLYGLY

Related Database Links:

PDB 2HPU 2HQ3
EMBL CAA75429
GB AAD09161 KDP72987
REF WP_029742711 WP_034799000

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks