BMRB Entry 5612
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5612
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Title: Structure of the Plant Defensin PhD1 from Petunia Hybrida PubMed: 12846570
Deposition date: 2002-12-02 Original release date: 2003-08-08
Authors: Janssen, B.; Schirra, H.; Lay, F.; Anderson, M.; Craik, D.
Citation: Janssen, B.; Schirra, H.; Lay, F.; Anderson, M.; Craik, D.. "Structure of Petunia hybrida Defensin 1, a Novel Plant Defensin with Five Disulfide Bonds" Biochemistry 42, 8214-8222 (2003).
Assembly members:
PhD1, polymer, 47 residues, Formula weight is not available
Natural source: Common Name: garden petunia Taxonomy ID: 4102 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Petunia x hybrida
Experimental source: Production method: .
Entity Sequences (FASTA):
PhD1: ATCKAECPTWDSVCINKKPC
VACCKKAKFSDGHCSKILRR
CLCTKEC
- coupling_constants
| Data type | Count |
| coupling constants | 13 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | floral defensin-like protein 1 | 1 |
Entities:
Entity 1, floral defensin-like protein 1 47 residues - Formula weight is not available
| 1 | ALA | THR | CYS | LYS | ALA | GLU | CYS | PRO | THR | TRP | ||||
| 2 | ASP | SER | VAL | CYS | ILE | ASN | LYS | LYS | PRO | CYS | ||||
| 3 | VAL | ALA | CYS | CYS | LYS | LYS | ALA | LYS | PHE | SER | ||||
| 4 | ASP | GLY | HIS | CYS | SER | LYS | ILE | LEU | ARG | ARG | ||||
| 5 | CYS | LEU | CYS | THR | LYS | GLU | CYS |
Samples:
sample_1: PhD1 0.94 mM; H2O 90%; D2O 10%
sample_2: PhD1 0.94 mM; H2O 99%; D2O 1%
sample_cond_1: ionic strength: 0.94 mM; pH: 3.1; pressure: 1 atm; temperature: 310 K
sample_cond_2: ionic strength: 0.94 mM; pH: 3.1; pressure: 1 atm; temperature: 280 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| E-COSY | not available | not available | not available |
| 2D NOESY | not available | not available | not available |
| DQF-COSY | not available | not available | not available |
Software:
XWINNMR v2.6 - collection, processing
CCNMR vglxccExplorer - data analysis
X-PLOR v3.851 - structure solution
CNS v1.1 - refinement, structure solution
NMR spectrometers:
- Bruker DMX 750 MHz