BMRB Entry 5703
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5703
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Title: SP phosphorothioate U6 RNA ISL PubMed: 12702812
Deposition date: 2003-02-17 Original release date: 2003-08-07
Authors: Reiter, Nicholas; Butcher, Samuel
Citation: Reiter, Nicholas; Nikstad, Laura; Allmann, Anne; Johnson, Randal; Butcher, Samuel. "Structure of the U6 RNA Intramolecular Stem-loop Harboring an SP-phosphorothioate Modification" RNA 9, 533-542 (2003).
Assembly members:
U6 snRNA, polymer, 24 residues, Formula weight is not available
Natural source: Common Name: Baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
U6 snRNA: GGUUCCCCUGCAUAAGGAXG
AACC
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 66 |
31P chemical shifts | 1 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | U6 snRNA | 1 |
Entities:
Entity 1, U6 snRNA 24 residues - Formula weight is not available
1 | G | G | U | U | C | C | C | C | U | G | ||||
2 | C | A | U | A | A | G | G | A | SSU | G | ||||
3 | A | A | C | C |
Samples:
sample_1: U6 snRNA 1 mM; NaCl 50 mM; H2O 90%; D2O 10%
sample_2: U6 snRNA 1 mM; NaCl 50 mM; D2O 100%
sample_3: U6 snRNA 1 mM; NaCl 50 mM; D2O 100%; Pf1 phage 17 mg/mL
cond_1: ionic strength: 0.05 M; pH: 7.0; temperature: 283 K
cond_2: ionic strength: 0.05 M; pH: 7.0; temperature: 303 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|
Software:
xwinnmr v2.6 -
SPARKY v2.6 -
NMR spectrometers:
- Bruker DMX 750 MHz
- Bruker DMX 600 MHz
- Bruker DMX 500 MHz
- Bruker DMX 400 MHz