BMRB Entry 5712
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5712
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Title: Backbone resonance assignment for human eIF4E in complex with m7GDP and a 17-amino acid peptide derived from human eIF4G
Deposition date: 2003-03-02 Original release date: 2006-05-30
Authors: Miura, Takaaki; Shiratori, Yasuhiko; Shimma, Nobuo
Citation: Miura, Takaaki; Shiratori, Yasuhiko; Shimma, Nobuo. "Letter to the Editor: Backbone resonance assignment of human eukaryotic translation initiation factor 4E (eIF4E) in complex with 7-methylguanosine diphosphate (m7GDP) and a 17-amino acid peptide derived from human eIF4GII " J. Biomol. NMR 27, 279-280 (2003).
Assembly members:
translation initiation factor 4E, polymer, 217 residues, 25097 Da.
eIF4E binding region, polymer, 17 residues, Formula weight is not available
M7G, non-polymer, 459.243 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
translation initiation factor 4E: MATVEPETTPTPNPPTTEEE
KTESNQEVANPEHYIKHPLQ
NRWALWFFKNDKSKTWQANL
RLISKFDTVEDFWALYNHIQ
LSSNLMPGCDYSLFKDGIEP
MWEDEKNKRGGRWLITLNKQ
QRRSDLDRFWLETLLCLIGE
SFDDYSDDVCGAVVNVRAKG
DKIAIWTTECENREAVTHIG
RVYKERLGLPPKIVIGYQSH
ADTATKSGSTTKNRFVV
eIF4E binding region: KKQYDREFLLDFQFMPA
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 197 |
| 13C chemical shifts | 606 |
| 15N chemical shifts | 197 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | eif4e monomer | 1 |
| 2 | eIF4E binding region | 2 |
| 3 | 7-methylguanosine diphosphate | 3 |
Entities:
Entity 1, eif4e monomer 217 residues - 25097 Da.
| 1 | MET | ALA | THR | VAL | GLU | PRO | GLU | THR | THR | PRO | ||||
| 2 | THR | PRO | ASN | PRO | PRO | THR | THR | GLU | GLU | GLU | ||||
| 3 | LYS | THR | GLU | SER | ASN | GLN | GLU | VAL | ALA | ASN | ||||
| 4 | PRO | GLU | HIS | TYR | ILE | LYS | HIS | PRO | LEU | GLN | ||||
| 5 | ASN | ARG | TRP | ALA | LEU | TRP | PHE | PHE | LYS | ASN | ||||
| 6 | ASP | LYS | SER | LYS | THR | TRP | GLN | ALA | ASN | LEU | ||||
| 7 | ARG | LEU | ILE | SER | LYS | PHE | ASP | THR | VAL | GLU | ||||
| 8 | ASP | PHE | TRP | ALA | LEU | TYR | ASN | HIS | ILE | GLN | ||||
| 9 | LEU | SER | SER | ASN | LEU | MET | PRO | GLY | CYS | ASP | ||||
| 10 | TYR | SER | LEU | PHE | LYS | ASP | GLY | ILE | GLU | PRO | ||||
| 11 | MET | TRP | GLU | ASP | GLU | LYS | ASN | LYS | ARG | GLY | ||||
| 12 | GLY | ARG | TRP | LEU | ILE | THR | LEU | ASN | LYS | GLN | ||||
| 13 | GLN | ARG | ARG | SER | ASP | LEU | ASP | ARG | PHE | TRP | ||||
| 14 | LEU | GLU | THR | LEU | LEU | CYS | LEU | ILE | GLY | GLU | ||||
| 15 | SER | PHE | ASP | ASP | TYR | SER | ASP | ASP | VAL | CYS | ||||
| 16 | GLY | ALA | VAL | VAL | ASN | VAL | ARG | ALA | LYS | GLY | ||||
| 17 | ASP | LYS | ILE | ALA | ILE | TRP | THR | THR | GLU | CYS | ||||
| 18 | GLU | ASN | ARG | GLU | ALA | VAL | THR | HIS | ILE | GLY | ||||
| 19 | ARG | VAL | TYR | LYS | GLU | ARG | LEU | GLY | LEU | PRO | ||||
| 20 | PRO | LYS | ILE | VAL | ILE | GLY | TYR | GLN | SER | HIS | ||||
| 21 | ALA | ASP | THR | ALA | THR | LYS | SER | GLY | SER | THR | ||||
| 22 | THR | LYS | ASN | ARG | PHE | VAL | VAL |
Entity 2, eIF4E binding region 17 residues - Formula weight is not available
| 1 | LYS | LYS | GLN | TYR | ASP | ARG | GLU | PHE | LEU | LEU | ||||
| 2 | ASP | PHE | GLN | PHE | MET | PRO | ALA |
Entity 3, 7-methylguanosine diphosphate - C11 H19 N5 O11 P2 - 459.243 Da.
| 1 | M7G |
Samples:
sample_1: translation initiation factor 4E, [U-85% 2H;U-95% 13C; U-95% 15N], 0.8 mM; eIF4E binding region 0.8 mM; ammonium acetate 75 mM; ammonium sulfate 100 mM
Ex-cond_1: pH: 6.8; temperature: 298 K; ionic strength: 0.65 M
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| TROSY-HNCO | sample_1 | not available | Ex-cond_1 |
| TROSY-HNCA | sample_1 | not available | Ex-cond_1 |
| TROSY-HN(CO)CA | sample_1 | not available | Ex-cond_1 |
| TROSY-HNCACB | sample_1 | not available | Ex-cond_1 |
| TROSY-HN(CO)CACB | sample_1 | not available | Ex-cond_1 |
| 1H-15N-NOESY | sample_1 | not available | Ex-cond_1 |
Software:
No software information available
NMR spectrometers:
- Bruker Avance 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAD92356 BAE02210 BAG57978 BAE02210 BAG57978 BAD92356 |
| GB | AAC02903 AAH94683 AAI36644 AAR32992 EAW94955 |
| REF | NP_001075231 NP_001185730 NP_001185731 NP_003751 XP_002750436 XP_423296 XP_001165083 XP_001377349 NP_001075231 NP_003751 |
| SP | O43432 |
| SWISS-PROT | O43432 |
| GenBank | EAW94955 EAW94956 AAH94683 AAR32992 AAC02903 |
| EMBL | CAI14553 CAI12534 CAI12535 CAI12155 CAI12158 |
| AlphaFold | O43432 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts