BMRB Entry 5754
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5754
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Title: 1H, 13C and 15N backbone resonance assignment of the VASP EVH1 domain PubMed: 12913419
Deposition date: 2003-03-24 Original release date: 2004-04-07
Authors: Vogtherr, Martin; Grimme, Susanne; Pescatore, Barbara; Schwalbe, Harald
Citation: Vogtherr, Martin; Grimme, Susanne; Pescatore, Barbara; Schwalbe, Harald. "Letter to the Editor: 1H, 13C and 15N Backbone Resonance Assignment of the VASP EVH1 Domain" J. Biomol. NMR 27, 189-190 (2003).
Assembly members:
Human VASP Ena-Vasp homology domain 1, polymer, 115 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Human VASP Ena-Vasp homology domain 1: MSETVICSSRATVMLYDDGN
KRWLPAGTGPQAFSRVQIYH
NPTANSFRVVGRKMQPDQQV
VINCAIVRGVKYNQATPNFH
QWRDARQVWGLNFGSKEDAA
QFAAGMASALEALEG
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 110 |
| 1H chemical shifts | 460 |
| 13C chemical shifts | 332 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | VASP EVH1 domain | 1 |
Entities:
Entity 1, VASP EVH1 domain 115 residues - Formula weight is not available
| 1 | MET | SER | GLU | THR | VAL | ILE | CYS | SER | SER | ARG | ||||
| 2 | ALA | THR | VAL | MET | LEU | TYR | ASP | ASP | GLY | ASN | ||||
| 3 | LYS | ARG | TRP | LEU | PRO | ALA | GLY | THR | GLY | PRO | ||||
| 4 | GLN | ALA | PHE | SER | ARG | VAL | GLN | ILE | TYR | HIS | ||||
| 5 | ASN | PRO | THR | ALA | ASN | SER | PHE | ARG | VAL | VAL | ||||
| 6 | GLY | ARG | LYS | MET | GLN | PRO | ASP | GLN | GLN | VAL | ||||
| 7 | VAL | ILE | ASN | CYS | ALA | ILE | VAL | ARG | GLY | VAL | ||||
| 8 | LYS | TYR | ASN | GLN | ALA | THR | PRO | ASN | PHE | HIS | ||||
| 9 | GLN | TRP | ARG | ASP | ALA | ARG | GLN | VAL | TRP | GLY | ||||
| 10 | LEU | ASN | PHE | GLY | SER | LYS | GLU | ASP | ALA | ALA | ||||
| 11 | GLN | PHE | ALA | ALA | GLY | MET | ALA | SER | ALA | LEU | ||||
| 12 | GLU | ALA | LEU | GLU | GLY |
Samples:
sample_1: Human VASP Ena-Vasp homology domain 1, [U-13C; U-15N], 1.0 mM; DTT 10 mM; KCl 50 mM; KPi 20 mM
sample_2: Human VASP Ena-Vasp homology domain 1, [U-15N], 1.0 mM; DTT 10 mM; KCl 50 mM; KPi 20 mM
Ex-cond_1: pH: 6.5; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| HNCO | not available | not available | Ex-cond_1 |
| HN(CA)CO | not available | not available | Ex-cond_1 |
| CBCA(CO)NH | not available | not available | Ex-cond_1 |
| HNCACB | not available | not available | Ex-cond_1 |
| HCC(CO)NH-TOCSY | not available | not available | Ex-cond_1 |
Software:
xwinnmr v2.9 - acqusition, processing
xeasy v1.3.14 - peak assignment
talos v1999.019.15.47 - assignment, structure validation
NMR spectrometers:
- Bruker DRX 800 MHz
Related Database Links:
| BMRB | 18569 |
| PDB | |
| DBJ | BAG37336 BAJ21098 |
| EMBL | CAA67147 CAA86523 |
| GB | AAH26019 AAH38224 AIC59345 EAW57362 EAW57363 |
| REF | NP_003361 XP_001106536 XP_002829453 XP_003277690 XP_003817563 |
| SP | P50552 |
| AlphaFold | P50552 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts