BMRB Entry 5766
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5766
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NMR-STAR v3 text file.
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Title: Solution Structure of the Lyase Domain of Human DNA Polymerase Lambda PubMed: 12911298
Deposition date: 2003-04-11 Original release date: 2003-09-08
Authors: DeRose, E.; Kirby, T.; Mueller, G.; Bebenek, K.; Garcia-Diaz, M.; Blanco, L.; Kunkel, T.; London, R.
Citation: DeRose, E.; Kirby, T.; Mueller, G.; Bebenek, K.; Garcia-Diaz, M.; Blanco, L.; Kunkel, T.; London, R.. "Solution Structure of the Lyase Domain of Human DNA Polymerase Lambda" Biochemistry 42, 9564-9574 (2003).
Assembly members:
50S Ribosomal Protein L18, polymer, 87 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: PET30A(+)
Entity Sequences (FASTA):
50S Ribosomal Protein L18: MAQPSSQKATNHNLHITEKL
EVLAKAYSVQGDKWRALGYA
KAINALKSFHKPVTSYQEAC
SIPGIGKRMAEKIIEILESG
HLRKLDH
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 440 |
| 13C chemical shifts | 328 |
| 15N chemical shifts | 76 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | 50S Ribosomal Protein L18 | 1 |
Entities:
Entity 1, 50S Ribosomal Protein L18 87 residues - Formula weight is not available
| 1 | MET | ALA | GLN | PRO | SER | SER | GLN | LYS | ALA | THR | ||||
| 2 | ASN | HIS | ASN | LEU | HIS | ILE | THR | GLU | LYS | LEU | ||||
| 3 | GLU | VAL | LEU | ALA | LYS | ALA | TYR | SER | VAL | GLN | ||||
| 4 | GLY | ASP | LYS | TRP | ARG | ALA | LEU | GLY | TYR | ALA | ||||
| 5 | LYS | ALA | ILE | ASN | ALA | LEU | LYS | SER | PHE | HIS | ||||
| 6 | LYS | PRO | VAL | THR | SER | TYR | GLN | GLU | ALA | CYS | ||||
| 7 | SER | ILE | PRO | GLY | ILE | GLY | LYS | ARG | MET | ALA | ||||
| 8 | GLU | LYS | ILE | ILE | GLU | ILE | LEU | GLU | SER | GLY | ||||
| 9 | HIS | LEU | ARG | LYS | LEU | ASP | HIS |
Samples:
sample_1: 50S Ribosomal Protein L18, [U-15N; U-13C], 2 mM; Tris-d11 5 mM; NaCl 100 mM; DTT 1 mM; NaN3 5 mM; H2O 90%; D2O 10%
sample_cond_1: pH: 7; temperature: 298 K; ionic strength: 110 mM; pressure: 1 atm
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 13C-separated NOESY | not available | not available | not available |
| 3D 15N-separated NOESY | not available | not available | not available |
Software:
VNMR v6.1C - collection
NMRPipe v2.1 - processing
NMRView v5.0.4 - data analysis
CNS v1.0 - structure solution
ARIA v1.1 - structure solution, refinement
NMR spectrometers:
- Varian INOVA 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAB13852 BAB14050 BAE02437 BAG10573 BAK63678 |
| EMBL | CAB65074 |
| GB | AAF27541 AAG22519 AAH68529 AAM77696 ADQ01128 |
| REF | NP_001167555 NP_001253835 NP_001267179 NP_001270218 NP_037406 |
| SP | Q4R380 Q9UGP5 |
| AlphaFold | Q4R380 Q9UGP5 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts