BMRB Entry 5790
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5790
MolProbity Validation Chart
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NMR-STAR v3 text file.
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Title: Solution structure of the Hypothetical protein AQ_1857 from Aquifex aeolicus: Northeast Structural Genomics Consortium Target QR6 PubMed: 14997575
Deposition date: 2003-05-01 Original release date: 2004-03-15
Authors: Xu, D.; Liu, G.; Xiao, R.; Acton, T.; Montelione, G.; Szyperski, T.
Citation: Xu, D.; Liu, G.; Xiao, R.; Acton, T.; Goldsmith-Fischman, S.; Honig, B.; Montelione, G.; Szyperski, T.. "NMR structure of the hypothetical protein AQ-1857 encoded by the Y157 gene from Aquifex aeolicus reveals a novel protein fold. " Proteins 54, 794-796 (2004).
Assembly members:
Hypothetical protein AQ_1857, polymer, 124 residues, Formula weight is not available
Natural source: Common Name: Aquifex aeolicus Taxonomy ID: 63363 Superkingdom: Eubacteria Kingdom: not available Genus/species: Aquifex aeolicus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: PET21
Entity Sequences (FASTA):
Hypothetical protein AQ_1857: MQEQAQQFIFKVTDKAVEEI
KKVAQENNIENPILRIRVVP
GGCSGFQYAMGFDDTVEEGD
HVFEYDGVKVVIDPFSMPYV
NGAELDYVVDFMGGGFTIRN
PNATGSCGCGSSFSCGLEHH
HHHH
- coupling_constants
| Data type | Count |
| coupling constants | 50 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Glutamate receptor interacting protein | 1 |
Entities:
Entity 1, Glutamate receptor interacting protein 124 residues - Formula weight is not available
| 1 | MET | GLN | GLU | GLN | ALA | GLN | GLN | PHE | ILE | PHE | ||||
| 2 | LYS | VAL | THR | ASP | LYS | ALA | VAL | GLU | GLU | ILE | ||||
| 3 | LYS | LYS | VAL | ALA | GLN | GLU | ASN | ASN | ILE | GLU | ||||
| 4 | ASN | PRO | ILE | LEU | ARG | ILE | ARG | VAL | VAL | PRO | ||||
| 5 | GLY | GLY | CYS | SER | GLY | PHE | GLN | TYR | ALA | MET | ||||
| 6 | GLY | PHE | ASP | ASP | THR | VAL | GLU | GLU | GLY | ASP | ||||
| 7 | HIS | VAL | PHE | GLU | TYR | ASP | GLY | VAL | LYS | VAL | ||||
| 8 | VAL | ILE | ASP | PRO | PHE | SER | MET | PRO | TYR | VAL | ||||
| 9 | ASN | GLY | ALA | GLU | LEU | ASP | TYR | VAL | VAL | ASP | ||||
| 10 | PHE | MET | GLY | GLY | GLY | PHE | THR | ILE | ARG | ASN | ||||
| 11 | PRO | ASN | ALA | THR | GLY | SER | CYS | GLY | CYS | GLY | ||||
| 12 | SER | SER | PHE | SER | CYS | GLY | LEU | GLU | HIS | HIS | ||||
| 13 | HIS | HIS | HIS | HIS |
Samples:
sample_1: Hypothetical protein AQ_1857, [U-15N; U-13C], 1.08 mM; MES 20 mM; NaCl 50 mM; DTT 5 mM; D2O 5%; NaN3 0.02%
sample_2: Hypothetical protein AQ_1857, [U-15N; U-5% 13C], 1.01 mM; MES 20 mM; NaCl 100 mM; DTT 10 mM; D2O 5%; NaN3 0.02%
sample_cond_1: pH: 6.5; temperature: 293 K; pressure: 1 atm
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 13C-separated NOESY | not available | not available | not available |
| 3D 15N-separated NOESY | not available | not available | not available |
| HNHA | not available | not available | not available |
| ct-13C-HSQC | not available | not available | not available |
| 3D-HNNCACB | not available | not available | not available |
| 3D-RD-HABCABcoNHN | not available | not available | not available |
| 3D-RD-HCCH-COSY | not available | not available | not available |
Software:
DYANA v1.5 - structure solution
NMRPipe v1.0 - processing
XEASY v1.3.1 - data analysis
PROSA v5.0 - processing
CYANA v1.0.3 - structure solution, refinement
NMR spectrometers:
- Varian INOVA 750 MHz