BMRB Entry 5794
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5794
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Title: 1H, 13C, 15N Chemical Shift Assignments for the SH3-SH2 domain pair of the tyrosin kinase Lck PubMed: 14512743
Deposition date: 2003-05-10 Original release date: 2003-10-16
Authors: Schweimer, Kristian; Kiessling, Anke; Bauer, Finn; Hor, Simon; Hoffmann, Silke; Roesch, Paul; Sticht, Heinrich
Citation: Schweimer, Kristian; Kiessling, Anke; Bauer, Finn; Hor, Simon; Hoffmann, Silke; Roesch, Paul; Sticht, Heinrich. "Letter to the Editor: Sequence-specific 1H, 13C and 15N resonance assignments of the SH3-SH2 domain pair of the human tyrosine kinase Lck " J. Biomol. NMR 27, 405-406 (2003).
Assembly members:
Tyrosine kinase, polymer, 169 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Tyrosine kinase: ASPLQDNLVIALHSYEPSHD
GDLGFEKGEQLRILEQSGEW
WKAQSLTTGQEGFIPFNFVA
KANSLEPEPWFFKNLSRKDA
ERQLLAPGNTHGSFLIRESE
STAGSFSLSVRDFDQNQGEV
VKHYKIRNLDNGGFYISPRI
TFPGLHELVRHYTNASDGLC
TRLSRPCQT
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 981 |
| 13C chemical shifts | 618 |
| 15N chemical shifts | 156 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | LckSH32 | 1 |
Entities:
Entity 1, LckSH32 169 residues - Formula weight is not available
| 1 | ALA | SER | PRO | LEU | GLN | ASP | ASN | LEU | VAL | ILE | ||||
| 2 | ALA | LEU | HIS | SER | TYR | GLU | PRO | SER | HIS | ASP | ||||
| 3 | GLY | ASP | LEU | GLY | PHE | GLU | LYS | GLY | GLU | GLN | ||||
| 4 | LEU | ARG | ILE | LEU | GLU | GLN | SER | GLY | GLU | TRP | ||||
| 5 | TRP | LYS | ALA | GLN | SER | LEU | THR | THR | GLY | GLN | ||||
| 6 | GLU | GLY | PHE | ILE | PRO | PHE | ASN | PHE | VAL | ALA | ||||
| 7 | LYS | ALA | ASN | SER | LEU | GLU | PRO | GLU | PRO | TRP | ||||
| 8 | PHE | PHE | LYS | ASN | LEU | SER | ARG | LYS | ASP | ALA | ||||
| 9 | GLU | ARG | GLN | LEU | LEU | ALA | PRO | GLY | ASN | THR | ||||
| 10 | HIS | GLY | SER | PHE | LEU | ILE | ARG | GLU | SER | GLU | ||||
| 11 | SER | THR | ALA | GLY | SER | PHE | SER | LEU | SER | VAL | ||||
| 12 | ARG | ASP | PHE | ASP | GLN | ASN | GLN | GLY | GLU | VAL | ||||
| 13 | VAL | LYS | HIS | TYR | LYS | ILE | ARG | ASN | LEU | ASP | ||||
| 14 | ASN | GLY | GLY | PHE | TYR | ILE | SER | PRO | ARG | ILE | ||||
| 15 | THR | PHE | PRO | GLY | LEU | HIS | GLU | LEU | VAL | ARG | ||||
| 16 | HIS | TYR | THR | ASN | ALA | SER | ASP | GLY | LEU | CYS | ||||
| 17 | THR | ARG | LEU | SER | ARG | PRO | CYS | GLN | THR |
Samples:
sample_1: Tyrosine kinase, [U-95% 13C; U-95% 15N], 0.5 – 0.7 mM
Ex-cond_1: pH: 6.4; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N HSQC | not available | not available | not available |
| 1H-13C constant time HSQC | not available | not available | not available |
| HNCO | not available | not available | not available |
| HNCA | not available | not available | not available |
| HNCACB | not available | not available | not available |
| CBCA(CO)NH | not available | not available | not available |
| CCCONH-TOCSY | not available | not available | not available |
| HBHA(CO)NH | not available | not available | not available |
| HCCH-COSY | not available | not available | not available |
| HCCH-TOCSY | not available | not available | not available |
Software:
NMRView v5.0.4 - visual analysis of the NMR spectra
home written software - processing of the NMR raw data, automatic assignment of the backbone resonances
NMR spectrometers:
- Bruker DRX 600 MHz
- Bruker Avance 700 MHz
Related Database Links:
| PDB | |
| DBJ | BAC40086 BAG64189 BAI45973 |
| EMBL | CAA27234 CAA31884 CAA32211 CAC38871 CAC44027 |
| GB | AAA18225 AAA59502 AAB59674 AAC50287 AAF34794 |
| PRF | 1203381A 2201317A |
| REF | NP_001036236 NP_001094179 NP_001155904 NP_001155905 NP_005347 |
| SP | P06239 P06240 Q01621 Q5PXS1 Q95KR7 |
| TPE | CAD55807 |
| AlphaFold | P06239 P06240 Q01621 Q5PXS1 Q95KR7 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts