BMRB Entry 5816

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5816

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignment of The Dimer Yeast Peroxiredoxin YLR109w
Deposition date:
2003-06-04
Original release date:
2003-12-18
Authors:
Trivelli, Xavier; Krimm, Isabelle; Tsan, Pascale; Lancelin, Jean-Marc
Citation:

Citation: Trivelli, Xavier; Krimm, Isabelle; Verdoucq, Lionel; Chartier, Yvette; Tsan, Pascale; Meyer, Yves; Lancelin, Jean-Marc. "Letter to the Editor: 1H, 13C and 15N backbone resonance assignments of the dimeric yeast peroxiredoxin YLR109w"  J. Biomol. NMR 28, 95-96 (2004).

Assembly members:

Assembly members:
YLR109w, polymer, 176 residues, 19115 Da.

Natural source:

Natural source:   Common Name: Baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
YLR109w: MSDLVNKKFPAGDYKFQYIA ISQSDADSESCKMPQTVEWS KLISENKKVIITGAPAAFSP TCTVSHIPGYINYLDELVKE KEVDQVIVVTVDNPFANQAW AKSLGVKDTTHIKFASDPGC AFTKSIGFELAVGDGVYWSG RWAMVVENGIVTYAAKETNP GTDVTVSSVESVLAHL

Data sets:
Data typeCount
13C chemical shifts513
15N chemical shifts162
1H chemical shifts168

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1YLR109w subunit 11
2YLR109w subunit 21

Entities:

Entity 1, YLR109w subunit 1 176 residues - 19115 Da.

1   METSERASPLEUVALASNLYSLYSPHEPRO
2   ALAGLYASPTYRLYSPHEGLNTYRILEALA
3   ILESERGLNSERASPALAASPSERGLUSER
4   CYSLYSMETPROGLNTHRVALGLUTRPSER
5   LYSLEUILESERGLUASNLYSLYSVALILE
6   ILETHRGLYALAPROALAALAPHESERPRO
7   THRCYSTHRVALSERHISILEPROGLYTYR
8   ILEASNTYRLEUASPGLULEUVALLYSGLU
9   LYSGLUVALASPGLNVALILEVALVALTHR
10   VALASPASNPROPHEALAASNGLNALATRP
11   ALALYSSERLEUGLYVALLYSASPTHRTHR
12   HISILELYSPHEALASERASPPROGLYCYS
13   ALAPHETHRLYSSERILEGLYPHEGLULEU
14   ALAVALGLYASPGLYVALTYRTRPSERGLY
15   ARGTRPALAMETVALVALGLUASNGLYILE
16   VALTHRTYRALAALALYSGLUTHRASNPRO
17   GLYTHRASPVALTHRVALSERSERVALGLU
18   SERVALLEUALAHISLEU

Samples:

sample_1: YLR109w, [U-50% 2H; U-13C; U-15N], 2.0 mM; DTT 5.0 mM

sample_2: YLR109w, [U-15N], 2.0 mM; DTT 5.0 mM

cond_1: pH: 5.8; temperature: 311 K

Experiments:

NameSampleSample stateSample conditions
1H-15N NOESYnot availablenot availablecond_1
HNCOnot availablenot availablecond_1
MQ-HNCOCAnot availablenot availablecond_1
HN(CA)COnot availablenot availablecond_1
HNCAnot availablenot availablecond_1
HN(COCA)CBnot availablenot availablecond_1
HN(CA)CBnot availablenot availablecond_1
(H)C(CCO)NH-TOCSYnot availablenot availablecond_1

Software:

NMRPipe - processing

PIPP - peak picking

NMR spectrometers:

  • Varian INOVA_Unity+ 800 MHz
  • Varian INOVA_Unity+ 600 MHz
  • Bruker DRX 500 MHz

Related Database Links:

PDB
DBJ GAA24996
EMBL CAA61687 CAA97676 CAY81346
GB AAB67554 AHY78511 EDN59654 EDV09411 EDZ70648
REF NP_013210
SP P38013
TPG DAA09425
AlphaFold P38013

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks