BMRB Entry 5825
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5825
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Title: Sequence-specific 1H, 13C and 15N resonance assignments of the C-terminal domain of KaiA, a circadian clock protein (reduced form) PubMed: 14872133
Deposition date: 2003-06-08 Original release date: 2004-02-13
Authors: Vakonakis, Ioannis; LiWang, Andy
Citation: Vakonakis, Ioannis; LiWang, Andy. "Letter to the Editor: Sequence-specific 1H, 13C and 15N resonance assignments of the C-terminal domain of KaiA, a circadian clock protein " J. Biomol. NMR 28, 403-404 (2004).
Assembly members:
Thermosynechococcus KaiA, polymer, 107 residues, 12583 Da.
Natural source: Common Name: Thermosynechococcus elongatus Taxonomy ID: 146786 Superkingdom: Eubacteria Kingdom: not available Genus/species: Thermosynechococcus elongatus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: BL21(DE3)
Entity Sequences (FASTA):
Thermosynechococcus KaiA: AMARMSPADKRKLLDELRSI
YRTIVLEYFNTDAKVNERID
EFVSKAFFADISVSQVLEIH
VELMDTFSKQLKLEGRSEDI
LLDYRLTLIDVIAHLCEMYR
RSIPREV
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 95 |
| 13C chemical shifts | 177 |
| 15N chemical shifts | 95 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | ThKaiA180C subunit 1 | 1 |
| 2 | ThKaiA180C subunit 2 | 1 |
Entities:
Entity 1, ThKaiA180C subunit 1 107 residues - 12583 Da.
| 1 | ALA | MET | ALA | ARG | MET | SER | PRO | ALA | ASP | LYS | ||||
| 2 | ARG | LYS | LEU | LEU | ASP | GLU | LEU | ARG | SER | ILE | ||||
| 3 | TYR | ARG | THR | ILE | VAL | LEU | GLU | TYR | PHE | ASN | ||||
| 4 | THR | ASP | ALA | LYS | VAL | ASN | GLU | ARG | ILE | ASP | ||||
| 5 | GLU | PHE | VAL | SER | LYS | ALA | PHE | PHE | ALA | ASP | ||||
| 6 | ILE | SER | VAL | SER | GLN | VAL | LEU | GLU | ILE | HIS | ||||
| 7 | VAL | GLU | LEU | MET | ASP | THR | PHE | SER | LYS | GLN | ||||
| 8 | LEU | LYS | LEU | GLU | GLY | ARG | SER | GLU | ASP | ILE | ||||
| 9 | LEU | LEU | ASP | TYR | ARG | LEU | THR | LEU | ILE | ASP | ||||
| 10 | VAL | ILE | ALA | HIS | LEU | CYS | GLU | MET | TYR | ARG | ||||
| 11 | ARG | SER | ILE | PRO | ARG | GLU | VAL |
Samples:
sample_1: Thermosynechococcus KaiA, [U-99% 13C; U-99% 15N], 1.2 mM; Na2HPO4 20.0 mM; NaCl 20.0 mM
cond_1: pH: 7.0 na; temperature: 323 K; ionic strength: 0.15 M
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 15N HSQC | not available | not available | not available |
| CBCA(CO)NH | not available | not available | not available |
Software:
NMRPipe v2.1 Rev 2002.044.17.08 - data processing
PIPP v4.2.6 - data analysis
NMR spectrometers:
- Varian INOVA 600 MHz
Related Database Links:
| BMRB | 5824 |
| PDB | |
| DBJ | BAB85983 BAC08033 BAD21221 |
| REF | NP_681271 WP_011056332 |
| SP | Q6L8K1 Q79V62 |
| AlphaFold | Q6L8K1 Q79V62 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts