BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5827

Title: Backbone and sidechain heteronuclear resonance assignments and hyperfine nuclear magnetic resonance shifts in horse cytochrome c   PubMed: 12931009

Deposition date: 2003-06-10 Original release date: 2003-09-28

Authors: Liu, Weixia; Rumbley, Jon; Englander, S.; Wand, A.

Citation: Liu, Weixia; Rumbley, Jon; Englander, S.; Wand, A.. "Backbone and Side-chain Heteronuclear Resonance Assignments and Hyperfine NMR Shifts in Horse Cytochrome c"  Protein Sci. 12, 2104-2108 (2003).

Assembly members:
Horse cytochrome c, polymer, 104 residues, Formula weight is not available
HEM, non-polymer, 616.487 Da.

Natural source:   Common Name: 9796   Taxonomy ID: Horse   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Equus caballus

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Horse cytochrome c: GDVEKGKKIFVQKCAQCHTV EKGGKNKTGPNLNGLFGRKT GQAPGFTYTDANKNKGITWK EETLMEYLENPKKYIPGTKM IFAGIKKKTEREDLIAYLKK ATNE

Data sets:
Data typeCount
1H chemical shifts559
13C chemical shifts421
15N chemical shifts98

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1cytochrome c1
2heme2

Entities:

Entity 1, cytochrome c 104 residues - Formula weight is not available

1   GLYASPVALGLULYSGLYLYSLYSILEPHE
2   VALGLNLYSCYSALAGLNCYSHISTHRVAL
3   GLULYSGLYGLYLYSASNLYSTHRGLYPRO
4   ASNLEUASNGLYLEUPHEGLYARGLYSTHR
5   GLYGLNALAPROGLYPHETHRTYRTHRASP
6   ALAASNLYSASNLYSGLYILETHRTRPLYS
7   GLUGLUTHRLEUMETGLUTYRLEUGLUASN
8   PROLYSLYSTYRILEPROGLYTHRLYSMET
9   ILEPHEALAGLYILELYSLYSLYSTHRGLU
10   ARGGLUASPLEUILEALATYRLEULYSLYS
11   ALATHRASNGLU

Entity 2, heme - C34 H32 Fe N4 O4 - 616.487 Da.

1   HEM

Samples:

sample_1: Horse cytochrome c 1.0 mM

sample_cond_1: pH: 5.8; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
3D CBCA(CO)NHsample_1not availablesample_cond_1
3D HNCACBsample_1not availablesample_cond_1
3D HNCOsample_1not availablesample_cond_1
3D (H)CC(CO)NH_TOCSYsample_1not availablesample_cond_1
3D H(CC)(CO)NH_TOCSYsample_1not availablesample_cond_1

Software:

FELIX v98 -

NMR spectrometers:

  • Varian INOVA 750 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts