BMRB Entry 5835
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5835
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Title: The Solution Structure and Dynamics of Recombinant Onconases PubMed: 14645226
Deposition date: 2003-06-17 Original release date: 2004-03-07
Authors: Gorbatyuk, Vitaliy; Tsai, Chen-Kuon; Chang, Chi-Fon; Huang, Tai-huang
Citation: Gorbatyuk, Vitaliy; Tsai, Chen-Kuon; Chang, Chi-Fon; Huang, Tai-huang. "Effect of N-terminal and Met23 mutations on the structure and dynamics of onconase" J. Biol. Chem. 279, 5772-5780 (2004).
Assembly members:
(M-1,Q1,M23L)rONC, polymer, 105 residues, 11958 Da.
Natural source: Common Name: Northern leopard frog Taxonomy ID: 8404 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rana pipiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-11d
Entity Sequences (FASTA):
(M-1,Q1,M23L)rONC: MQDWLTFQKKHITNTRDVDC
DNILSTNLFHCKDKNTFIYS
RPEPVKAICKGIIASKNVLT
TSEFYLSDCNVTSRPCKYKL
KKSTNKFCVTCENQAPVHFV
GVGSC
- coupling_constants
| Data type | Count |
| coupling constants | 84 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | (M-1,Q1,M23L)rOnc | 1 |
Entities:
Entity 1, (M-1,Q1,M23L)rOnc 105 residues - 11958 Da.
| 1 | MET | GLN | ASP | TRP | LEU | THR | PHE | GLN | LYS | LYS | ||||
| 2 | HIS | ILE | THR | ASN | THR | ARG | ASP | VAL | ASP | CYS | ||||
| 3 | ASP | ASN | ILE | LEU | SER | THR | ASN | LEU | PHE | HIS | ||||
| 4 | CYS | LYS | ASP | LYS | ASN | THR | PHE | ILE | TYR | SER | ||||
| 5 | ARG | PRO | GLU | PRO | VAL | LYS | ALA | ILE | CYS | LYS | ||||
| 6 | GLY | ILE | ILE | ALA | SER | LYS | ASN | VAL | LEU | THR | ||||
| 7 | THR | SER | GLU | PHE | TYR | LEU | SER | ASP | CYS | ASN | ||||
| 8 | VAL | THR | SER | ARG | PRO | CYS | LYS | TYR | LYS | LEU | ||||
| 9 | LYS | LYS | SER | THR | ASN | LYS | PHE | CYS | VAL | THR | ||||
| 10 | CYS | GLU | ASN | GLN | ALA | PRO | VAL | HIS | PHE | VAL | ||||
| 11 | GLY | VAL | GLY | SER | CYS |
Samples:
sample_1: (M-1,Q1,M23L)rONC, [U-15N; U-13C], 1.5 mM
sample_conditions_set_1: pH*: 4.1; temperature: 310 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D HNHA | sample_1 | not available | sample_conditions_set_1 |
Software:
No software information available
NMR spectrometers:
- Bruker DRX 600 MHz