BMRB Entry 5889
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5889
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Title: 1H assignments for yeast nucleoporin Nsp1 residues 528-557 PubMed: 14556747
Deposition date: 2003-07-30 Original release date: 2003-12-19
Authors: Morrison, Jonathan; Yang, Ji-Chun; Stewart, Murray; Neuhaus, David
Citation: Morrison, Jonathan; Yang, Ji-Chun; Stewart, Murray; Neuhaus, David. "Solution NMR study of the interaction between NTF2 and nucleoporin FxFG repeats" J. Mol. Biol. 333, 587-603 (2003).
Assembly members:
nucleoporin Nsp1 528-557, polymer, 30 residues, Formula weight is not available
Natural source: Common Name: Baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
nucleoporin Nsp1 528-557: KPAFSFGAKANEKKESDESK
SAFSFGSKPT
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 142 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Nsp1 528-557 | 1 |
Entities:
Entity 1, Nsp1 528-557 30 residues - Formula weight is not available
| 1 | LYS | PRO | ALA | PHE | SER | PHE | GLY | ALA | LYS | ALA | |
| 2 | ASN | GLU | LYS | LYS | GLU | SER | ASP | GLU | SER | LYS | |
| 3 | SER | ALA | PHE | SER | PHE | GLY | SER | LYS | PRO | THR |
Samples:
sample_1: nucleoporin Nsp1 528-5571 – 2 mM
Ex-cond_1: pH: 6.5; temperature: 300 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D (1H, 1H) NOESY | not available | not available | not available |
| 2D (1H, 1H) ROESY | not available | not available | not available |
| 2D (1H, 1H) TOCSY | not available | not available | not available |
Software:
xwin-nmr v2.6 - processing
SPARKY v3.91 - visualisation during assignment
NMR spectrometers:
- Bruker Avance 800 MHz
Related Database Links:
| PDB |