BMRB Entry 5924

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR5924
MolProbity Validation Chart

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Title:
NMR Solution Structure of the Focal Adhesion Targeting Domain (FAT) of Focal Adhesion Kinase (FAK) in Complex with a Paxillin LD Peptide: Evidence for a Two Site Binding Model
Deposition date:
2003-08-28
Original release date:
2004-03-02
Authors:
Gao, Guanghua; Prutzman, Kirk; King, Michelle; DeRose, Eugene; London, Robert; Schaller, Michael; Campbell, Sharon
Citation:

Citation: Gao, Guanghua; Prutzman, Kirk; King, Michelle; Scheswohl, D; DeRose, Eugene; London, Robert; Schaller, Michael; Campbell, Sharon. "NMR solution structure of the focal adhesion targeting domain of focal adhesion kinase in complex with a paxillin LD peptide: evidence for a two site binding model"  J. Biol. Chem. 279, 8441-8451 (2004).
PubMed: 14662767

Assembly members:

Assembly members:
FAT domain of Focal adhesion kinase (920-1053), polymer, 134 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Chicken   Taxonomy ID: 9031   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Gallus gallus

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
FAT domain of Focal adhesion kinase (920-1053): RSNDKVYENVTGLVKAVIEM SSKIQPAPPEEYVPMVKEVG LALRTLLATVDESLPVLPAS THREIEMAQKLLNSDLAELI NKMKLAQQYVMTSLQQEYKK QMLTAAHALAVDAKNLLDVI DQARLKMISQSRPH

Data sets:
Data typeCount
1H chemical shifts776
13C chemical shifts358
15N chemical shifts129

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1FAT of FAK1

Entities:

Entity 1, FAT of FAK 134 residues - Formula weight is not available

1   ARGSERASNASPLYSVALTYRGLUASNVAL
2   THRGLYLEUVALLYSALAVALILEGLUMET
3   SERSERLYSILEGLNPROALAPROPROGLU
4   GLUTYRVALPROMETVALLYSGLUVALGLY
5   LEUALALEUARGTHRLEULEUALATHRVAL
6   ASPGLUSERLEUPROVALLEUPROALASER
7   THRHISARGGLUILEGLUMETALAGLNLYS
8   LEULEUASNSERASPLEUALAGLULEUILE
9   ASNLYSMETLYSLEUALAGLNGLNTYRVAL
10   METTHRSERLEUGLNGLNGLUTYRLYSLYS
11   GLNMETLEUTHRALAALAHISALALEUALA
12   VALASPALALYSASNLEULEUASPVALILE
13   ASPGLNALAARGLEULYSMETILESERGLN
14   SERARGPROHIS

Samples:

sample_1: FAT domain of Focal adhesion kinase (920-1053), [U-13C; U-15N], 0.6 mM

expt_cond_1: pH: 6.0 na; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
3D simultaneous 13C/15N edited NOESYnot availablenot availablenot available
2D 1H-15N TROSY HSQCnot availablenot availablenot available
2D 1H-13C HSQCnot availablenot availablenot available
3D HNCAnot availablenot availablenot available
3D HN(CO)CAnot availablenot availablenot available
3D HNCACBnot availablenot availablenot available
3D CBCA(CO)NHnot availablenot availablenot available
3D HNCOnot availablenot availablenot available
3D HCCH-COSYnot availablenot availablenot available
3D HCCH-TOCSYnot availablenot availablenot available
3D H(CCO)-TOCSY-NNHnot availablenot availablenot available
3D (H)C(CO)-TOCSY-NNHnot availablenot availablenot available
2D (HB)CB(CGCDCE)HEnot availablenot availablenot available
2D (HB)CB(CGCD)HDnot availablenot availablenot available

Software:

NMRPIPE v2.1 -

NMRVIEW v5.0.4 -

NMR spectrometers:

  • Varian Inova 600 MHz
  • Varian Inova 800 MHz

Related Database Links:

BMRB 5266 5677
PDB
GB AAA48765 AAA48773 EMC80181 EOA99770 KFM11460
REF NP_990766 XP_002191290 XP_004174254 XP_005021656 XP_005021657
SP Q00944
AlphaFold Q00944

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks